He Songzhe, Li Zheng, Quan Meilian, Hong Zhu, Yang Qiu
Clin Lab. 2019 Aug 1;65(8). doi: 10.7754/Clin.Lab.2019.190125.
Parotid cyst is a common problem in patients treated by surgeons. However, Paracoccus yeei was isolated from an aerobic blood culture in a patient with parotid cyst as an unusual etiologic opportunistic agent.
Since old biochemical identification kits are not able to identify this species, MALDI-TOF MS correctly was recommended to identify this isolate. Its identity was confirmed by sequencing of the 16S rRNA gene.
The aligned sequences (16S rRNA gene) were used for a phylogenetic analysis (phylogenetic tree), which was produced using the BLAST pair-wise alignments. The sequence analysis determined that the best matches were with Paracoccus yeei.
Paracoccus yeei has been reported as a rare opportunistic human pathogen, we should actively com-municate to the clinic to improve the real positive rate.
腮腺囊肿是外科医生治疗患者时的常见问题。然而，在一名患有腮腺囊肿的患者的需氧血培养中分离出了耶氏副球菌，它是一种不常见的病原性机会致病菌。
由于旧的生化鉴定试剂盒无法鉴定该菌种，推荐使用基质辅助激光解吸电离飞行时间质谱（MALDI-TOF MS）正确鉴定该分离株。通过对16S rRNA基因进行测序确认其身份。
比对后的序列（16S rRNA基因）用于系统发育分析（系统发育树），该分析使用BLAST成对比对生成。序列分析确定最佳匹配对象是耶氏副球菌。
耶氏副球菌已被报道为一种罕见的人类机会致病菌，我们应积极与临床沟通以提高实际阳性率。
