Dominguez-Moñino Irene, Jurado Valme, Hermosin Bernardo, Saiz-Jimenez Cesareo
Instituto de Recursos Naturales y Agrobiologia, IRNAS-CSIC, Av. Reina Mercedes 10, 41012 Sevilla, Spain.
Int J Syst Evol Microbiol. 2016 Jun;66(6):2265-2270. doi: 10.1099/ijsem.0.001018. Epub 2016 Mar 11.
A Gram-stain-negative, aerobic, non-motile, non-spore-forming bacterium, strain 0511ARD5E5T, was isolated from an air sample collected in Ardales Cave (Malaga, Spain). Strain 0511ARD5E5T grew at 4-37 °C and in the presence of 0-4 % (w/v) NaCl [optimally at 25 °C and with 1 % (w/v) NaCl]. Cells were catalase- and oxidase-positive. The major respiratory quinone was ubiquinone-10. The predominant fatty acids were C18:1ω7c and C16:0. The DNA G+C content was 63.2 mol%. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain 0511ARD5E5T was a member of the genus Paracoccus and was related most closely to Paracoccus aminophilus DSM 8538T and Paracoccus marinus CIP 108500T (96.93 and 96.92 % similarity, respectively). Strain 0511ARD5E5T exhibited DNA-DNA relatedness of 47 % to P. aminophilus DSM 8538T and 31 % to P. marinus CIP 108500T. Chemotaxonomic, phenotypic and phylogenetic analyses indicated that strain 0511ARD5E5T represents a novel species of the genus Paracoccus, for which the name Paracoccus cavernae sp. nov. is proposed. The type strain is 0511ARD5E5T (=LMG 27962T=CECT 8482T).
从西班牙马拉加阿尔达莱斯洞穴采集的空气样本中分离出了一株革兰氏染色阴性、需氧、不运动、不形成芽孢的细菌，菌株0511ARD5E5T。菌株0511ARD5E5T在4-37°C以及0-4%（w/v）NaCl存在的条件下生长[最适生长温度为25°C，NaCl浓度为1%（w/v）]。细胞过氧化氢酶和氧化酶呈阳性。主要呼吸醌为泛醌-10。主要脂肪酸为C18:1ω7c和C16:0。DNA G+C含量为63.2 mol%。基于16S rRNA基因序列的系统发育分析表明，菌株0511ARD5E5T是副球菌属的成员，与嗜氨基副球菌DSM 8538T和海生副球菌CIP 108500T关系最为密切（相似性分别为96.93%和96.92%）。菌株0511ARD5E5T与嗜氨基副球菌DSM 8538T的DNA-DNA相关性为47%，与海生副球菌CIP 108500T的DNA-DNA相关性为31%。化学分类、表型和系统发育分析表明菌株0511ARD5E5T代表副球菌属的一个新物种，为此提出新名称洞穴副球菌（Paracoccus cavernae sp. nov.）。模式菌株为0511ARD5E5T（=LMG 27962T=CECT 8482T）。
