Xie Jing, Li Li, Shi Yurui, Chen Rongda, Liu Guiming, Wang Mengxue, Zheng Meizhu, Zhang Ning
Laboratory of Chemistry Analytical, Department of Chemical Analysis,, College of Changchun Normal University, Changchun, China.
Key Laboratory of Chinese Materia Medica (Ministry of Education),Department of Pharmaceutical Analysis, College of Jiamusi, Heilongjiang University of Chinese Medicine, Harbin, China.
Biomed Chromatogr. 2019 Dec;33(12):e4680. doi: 10.1002/bmc.4680. Epub 2019 Sep 8.
In this study, an accurate and reliable method of ultra-performance liquid chromatography coupled with a triple-quadrupole tandem mass spectrometry was firstly developed and fully validated for the simultaneous determination of epicatechin, neoastilbin, astilbin, isoastilbin, engeletin and resveratrol in rat plasma after administration of Smilacis glabrae Roxb. extract. Naringenin was used as an internal standard (IS). The analyte and IS were separated on a C column by gradient elution with a mobile phase of acetonitrile-0.3% acetic acid at a flow rate of 0.25 mL/min for a total run time of 8 min. The method was validated in terms of selectivity, linearity, precision, accuracy, extraction recovery, matrix effect and stability. The developed method was successfully applied to determine the main pharmacokinetic parameters of six components in rat plasma.
在本研究中，首次建立了一种准确可靠的超高效液相色谱-三重四极杆串联质谱法，并对其进行了全面验证，用于同时测定大鼠灌胃光叶菝葜提取物后血浆中表儿茶素、新异槲皮苷、异槲皮苷、异阿替林、恩格勒汀和白藜芦醇的含量。柚皮素用作内标（IS）。分析物和内标在C柱上通过梯度洗脱分离，流动相为乙腈-0.3%乙酸，流速为0.25 mL/min，总运行时间为8 min。该方法在选择性、线性、精密度、准确度、提取回收率、基质效应和稳定性方面进行了验证。所建立的方法成功应用于测定大鼠血浆中六种成分的主要药代动力学参数。
