Bozaoglu Kiymet, Gao Yujing, Stanley Edouard, Fanjul-Fernández Miriam, Brown Natasha J, Pope Kate, Green Cherie C, Vlahos Katerina, Sourris Koula, Bahlo Melanie, Delatycki Martin, Scheffer Ingrid, Lockhart Paul J
Bruce Lefroy Centre for Genetic Health Research, Murdoch Children's Research Institute, Melbourne, Victoria 3052, Australia; Department of Paediatrics, University of Melbourne, Parkville, Australia.
Department of Paediatrics, University of Melbourne, Parkville, Australia; Murdoch Children's Research Institute, Parkville, Australia; Department of Anatomy and Developmental Biology, Monash University, Clayton, Victoria 3800, Australia.
Stem Cell Res. 2019 Aug;39:101516. doi: 10.1016/j.scr.2019.101516. Epub 2019 Aug 1.
We have generated and characterized seven human induced pluripotent stem cell (iPSC) lines derived from peripheral blood mononuclear cells (PBMCs) from a single family, including unaffected and affected individuals clinically diagnosed with Autism Spectrum Disorder (ASD). The reprogramming of the PBMCs was performed using non-integrative Sendai virus containing the reprogramming factors POU5F1 (OCT4), SOX2, KLF4 and MYC. All iPSC lines exhibited a normal karyotype and pluripotency was validated by immunofluorescence, flow cytometry and their ability to differentiate into the three embryonic germ layers. These iPSC lines are a valuable resource to study the molecular mechanisms underlying ASD.
我们从一个家族的外周血单个核细胞（PBMC）中生成并鉴定了7个人诱导多能干细胞（iPSC）系，其中包括临床诊断为自闭症谱系障碍（ASD）的未受影响个体和受影响个体。使用含有重编程因子POU5F1（OCT4）、SOX2、KLF4和MYC的非整合仙台病毒对PBMC进行重编程。所有iPSC系均表现出正常的核型，通过免疫荧光、流式细胞术以及它们分化为三个胚胎胚层的能力验证了多能性。这些iPSC系是研究ASD潜在分子机制的宝贵资源。
