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Complete Genome Sequence of Dehalococcoides mccartyi Strain FL2, a Trichloroethene-Respiring Anaerobe Isolated from Pristine Freshwater Sediment.脱卤球菌属菌株FL2的全基因组序列,该菌株是从原始淡水沉积物中分离出的一种以三氯乙烯为呼吸底物的厌氧菌。
Microbiol Resour Announc. 2019 Aug 15;8(33):e00558-19. doi: 10.1128/MRA.00558-19.
2
Isolation and characterization of Dehalococcoides sp. strain FL2, a trichloroethene (TCE)- and 1,2-dichloroethene-respiring anaerobe.脱卤球菌属菌株FL2的分离与特性研究,该菌株为以三氯乙烯(TCE)和1,2 - 二氯乙烯为呼吸底物的厌氧菌。
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Genomic characterization of Dehalococcoides mccartyi strain 11a5 reveals a circular extrachromosomal genetic element and a new tetrachloroethene reductive dehalogenase gene.戴奥辛单胞菌 11a5 菌株的基因组特征揭示了一个环状染色体外遗传元件和一个新的四氯乙烯还原脱卤酶基因。
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Appl Environ Microbiol. 2004 Sep;70(9):5290-7. doi: 10.1128/AEM.70.9.5290-5297.2004.
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Dehalococcoides mccartyi gen. nov., sp. nov., obligately organohalide-respiring anaerobic bacteria relevant to halogen cycling and bioremediation, belong to a novel bacterial class, Dehalococcoidia classis nov., order Dehalococcoidales ord. nov. and family Dehalococcoidaceae fam. nov., within the phylum Chloroflexi.地杆菌属 mccartyi 属,新种,严格依赖有机卤化物进行呼吸的厌氧细菌,与卤代物循环和生物修复有关,属于新的细菌类群,脱卤球菌纲(Dehalococcoidia classis nov.),脱卤球菌目(Dehalococcoidales ord. nov.)和脱卤球菌科(Dehalococcoidaceae fam. nov.),在绿弯菌门(Chloroflexi)内。
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Dual Element (C/Cl) Isotope Analysis Indicates Distinct Mechanisms of Reductive Dehalogenation of Chlorinated Ethenes and Dichloroethane in Strain BTF08 With Defined Reductive Dehalogenase Inventories.双元素(碳/氯)同位素分析表明,具有特定还原脱卤酶清单的菌株BTF08中,氯乙烯和二氯乙烷的还原脱卤机制不同。
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本文引用的文献

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Purinyl-cobamide is a native prosthetic group of reductive dehalogenases.嘌呤基钴胺素是还原性脱卤酶的一种天然辅基。
Nat Chem Biol. 2018 Jan;14(1):8-14. doi: 10.1038/nchembio.2512. Epub 2017 Nov 6.
2
Complete Genome Sequence of Strain DMC, a Strictly Anaerobic Dichloromethane-Degrading Bacterium.严格厌氧的二氯甲烷降解菌DMC菌株的全基因组序列
Genome Announc. 2017 Sep 14;5(37):e00897-17. doi: 10.1128/genomeA.00897-17.
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Canu: scalable and accurate long-read assembly via adaptive -mer weighting and repeat separation.Canu:通过自适应k-mer加权和重复序列分离实现可扩展且准确的长读长序列拼接
Genome Res. 2017 May;27(5):722-736. doi: 10.1101/gr.215087.116. Epub 2017 Mar 15.
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NCBI prokaryotic genome annotation pipeline.美国国立生物技术信息中心原核生物基因组注释管道
Nucleic Acids Res. 2016 Aug 19;44(14):6614-24. doi: 10.1093/nar/gkw569. Epub 2016 Jun 24.
5
Identification of a multi-protein reductive dehalogenase complex in Dehalococcoides mccartyi strain CBDB1 suggests a protein-dependent respiratory electron transport chain obviating quinone involvement.在嗜盐脱卤球菌菌株CBDB1中鉴定出一种多蛋白还原脱卤酶复合物,这表明存在一种不依赖醌参与的蛋白质依赖性呼吸电子传递链。
Environ Microbiol. 2016 Sep;18(9):3044-56. doi: 10.1111/1462-2920.13200. Epub 2016 Feb 16.
6
Unexpected specificity of interspecies cobamide transfer from Geobacter spp. to organohalide-respiring Dehalococcoides mccartyi strains.意想不到的种间钴胺素转移特异性:从 Geobacter spp. 到有机卤代物呼吸的 Dehalococcoides mccartyi 菌株。
Appl Environ Microbiol. 2012 Sep;78(18):6630-6. doi: 10.1128/AEM.01535-12. Epub 2012 Jul 6.
7
Dehalococcoides mccartyi gen. nov., sp. nov., obligately organohalide-respiring anaerobic bacteria relevant to halogen cycling and bioremediation, belong to a novel bacterial class, Dehalococcoidia classis nov., order Dehalococcoidales ord. nov. and family Dehalococcoidaceae fam. nov., within the phylum Chloroflexi.地杆菌属 mccartyi 属,新种,严格依赖有机卤化物进行呼吸的厌氧细菌,与卤代物循环和生物修复有关,属于新的细菌类群,脱卤球菌纲(Dehalococcoidia classis nov.),脱卤球菌目(Dehalococcoidales ord. nov.)和脱卤球菌科(Dehalococcoidaceae fam. nov.),在绿弯菌门(Chloroflexi)内。
Int J Syst Evol Microbiol. 2013 Feb;63(Pt 2):625-635. doi: 10.1099/ijs.0.034926-0. Epub 2012 Apr 27.
8
PHAST: a fast phage search tool.PHAST:一种快速噬菌体搜索工具。
Nucleic Acids Res. 2011 Jul;39(Web Server issue):W347-52. doi: 10.1093/nar/gkr485. Epub 2011 Jun 14.
9
Localized plasticity in the streamlined genomes of vinyl chloride respiring Dehalococcoides.氯乙烯呼吸脱卤菌流线型基因组中的局部可塑性。
PLoS Genet. 2009 Nov;5(11):e1000714. doi: 10.1371/journal.pgen.1000714. Epub 2009 Nov 6.
10
Isolation and characterization of Dehalococcoides sp. strain FL2, a trichloroethene (TCE)- and 1,2-dichloroethene-respiring anaerobe.脱卤球菌属菌株FL2的分离与特性研究,该菌株为以三氯乙烯(TCE)和1,2 - 二氯乙烯为呼吸底物的厌氧菌。
Environ Microbiol. 2005 Sep;7(9):1442-50. doi: 10.1111/j.1462-2920.2005.00830.x.

脱卤球菌属菌株FL2的全基因组序列,该菌株是从原始淡水沉积物中分离出的一种以三氯乙烯为呼吸底物的厌氧菌。

Complete Genome Sequence of Dehalococcoides mccartyi Strain FL2, a Trichloroethene-Respiring Anaerobe Isolated from Pristine Freshwater Sediment.

作者信息

Yan Jun, Yang Yi, Li Xiuying, Löffler Frank E

机构信息

Key Laboratory of Pollution Ecology and Environmental Engineering, Institute of Applied Ecology, Chinese Academy of Sciences, Shenyang, Liaoning, China.

Department of Microbiology, University of Tennessee, Knoxville, Tennessee, USA.

出版信息

Microbiol Resour Announc. 2019 Aug 15;8(33):e00558-19. doi: 10.1128/MRA.00558-19.

DOI:10.1128/MRA.00558-19
PMID:31416865
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC6696640/
Abstract

strain FL2 couples growth to hydrogen oxidation and reductive dechlorination of trichloroethene and - and -1,2-dichloroethenes. Strain FL2 has a 1.42-Mb genome with a G+C content of 47.0% and carries 1,465 protein-coding sequences, including 24 reductive dehalogenase genes.

摘要

菌株FL2将生长与氢气氧化以及三氯乙烯和1,2 - 二氯乙烯的还原脱氯作用联系起来。菌株FL2拥有一个1.42兆碱基的基因组,G+C含量为47.0%,并携带1465个蛋白质编码序列,其中包括24个还原脱卤酶基因。