Lipoprotein SPD_1609 of Promotes Adherence and Invasion to Epithelial Cells Contributing to Bacterial Virulence.

In most bacteria, iron plays a vital role in pathogenesis processes. To support survival and infection, has evolved three ABC transporters, PiaABC, PiuABC, and PitABC. Moreover, indirect evidence supports that operon 804 may be a novel ABC transporter in . We have recently described the identification of lipoprotein SPD_1609 in operon 804; however, whether the SPD_1609 protein affects the virulence of has not yet been studied. In the present work, alignment analysis showed that lipoprotein SPD_1609 is conserved in a variety of gram-positive bacteria, and deletion of the gene led to a reduction in adherence and invasion of to host cells. RT-qPCR assays indicated that deletion of the gene resulted in decreased expression of genes involved in colonization. Furthermore, decreased virulence in a mouse bacteremia infection model caused by the loss of the lipoprotein encoded by was also demonstrated. Overall, these data provide insights into the functional role of lipoprotein SPD_1609 in pneumococcal pathogenesis, suggesting its possibility to be developed as a novel vaccine candidate.