College of Environment, Zhejiang University of Technology, Hangzhou 310032, PR China.
College of Environment, Zhejiang University of Technology, Hangzhou 310032, PR China.
Environ Pollut. 2019 Nov;254(Pt A):113019. doi: 10.1016/j.envpol.2019.113019. Epub 2019 Aug 8.
Microcystis aeruginosa is one of the main species of cyanobacteria that causes water blooms. M. aeruginosa can release into the water several types of microcystins (MCs), which are harmful to aquatic organisms and even humans. However, few studies have investigated the hepatotoxicity of M. aeruginosa itself in zebrafish in environments that simulate natural aquatic systems. The objective of this study was to evaluate the hepatotoxicity of M. aeruginosa in adult zebrafish (Danio rerio) after short-term (96 h) exposure and to elucidate the potential underlying mechanisms. Distinct histological changes in the liver, such as enlargement of the peripheral nuclei and sinusoids and the appearance of fibroblasts, were observed in zebrafish grown in M. aeruginosa culture. In addition, antioxidant enzyme activity was activated and protein phosphatase (PP) activity was significantly decreased with increasing microalgal density. A proteomic analysis revealed alterations in a number of protein pathways, including ribosome translation, immune response, energy metabolism and oxidative phosphorylation pathways. Western blot and real-time PCR analyses confirmed the results of the proteomic analysis. All results indicated that M. aeruginosa could disrupt hepatic functions in adult zebrafish, thus highlighting the necessity of ecotoxicity assessments for M. aeruginosa at environmentally relevant densities.
铜绿微囊藻是引发水华的主要蓝藻物种之一。铜绿微囊藻可以向水中释放多种类型的微囊藻毒素（MCs），这些毒素对水生生物甚至人类都有害。然而，很少有研究调查过在模拟自然水生系统的环境中，铜绿微囊藻本身对成年斑马鱼（Danio rerio）的肝毒性。本研究旨在评估短期（96 小时）暴露后铜绿微囊藻对成年斑马鱼的肝毒性，并阐明潜在的机制。在生长有铜绿微囊藻的斑马鱼中观察到明显的肝脏组织学变化，如外围核和窦间隙增大以及成纤维细胞出现。此外，随着微藻密度的增加，抗氧化酶活性被激活，蛋白磷酸酶（PP）活性显著降低。蛋白质组学分析显示，包括核糖体翻译、免疫反应、能量代谢和氧化磷酸化途径在内的许多蛋白途径发生了改变。Western blot 和实时 PCR 分析证实了蛋白质组学分析的结果。所有结果表明，铜绿微囊藻可以破坏成年斑马鱼的肝脏功能，因此强调了在环境相关密度下对铜绿微囊藻进行生态毒性评估的必要性。
