College of Environment, Zhejiang University of Technology, Hangzhou 310014, China.
College of Environment, Zhejiang University of Technology, Hangzhou 310014, China.
Sci Total Environ. 2021 Nov 15;795:148865. doi: 10.1016/j.scitotenv.2021.148865. Epub 2021 Jul 3.
Cyanobacterial blooms constitute a global ecological problem that can seriously threaten human health. One of the most common bloom-forming cyanobacteria in freshwater is Microcystis aeruginosa, whose secretion of toxic substances (microcystins, MCs) have strong liver toxicity and endanger the health of exposed people through contaminated aquatic products and drinking water. However, few studies on the neurotoxicity of M. aeruginosa to zebrafish have simulated the process of an actual cyanobacterial bloom. In this study, we used the zebrafish (Danio rerio) as an effective model organism to study the acute neurotoxicity of M. aeruginosa, and to clarify its principal mechanism of action. A total of 82 upregulated and 26 downregulated proteins were detected by quantitative proteomics analysis in zebrafish brain after exposure to M. aeruginosa. Intriguingly, these proteins with changed expression were related to Synaptic vesicle cycle and terpenoid skeleton biosynthesis pathway, such as ACAT, STX1A, and V-ATPase. The obtained results uniformly indicated that the neurotoxicity of M. aeruginosa seriously damaged the neurotransmitter conduction in the nervous system and brain information storage and transmission of zebrafish and makes it more susceptible to neurological diseases. Our study provides a new perspective on the neurotoxicity risk of cyanobacterial blooms.
蓝藻水华是一个全球性的生态问题，可能严重威胁人类健康。淡水环境中最常见的蓝藻水华物种之一是铜绿微囊藻，其分泌的有毒物质（微囊藻毒素，MCs）具有很强的肝毒性，通过受污染的水产品和饮用水危害暴露人群的健康。然而，很少有研究模拟实际蓝藻水华过程来研究铜绿微囊藻对斑马鱼的神经毒性。在这项研究中，我们使用斑马鱼（Danio rerio）作为一种有效的模式生物来研究铜绿微囊藻的急性神经毒性，并阐明其主要作用机制。定量蛋白质组学分析显示，暴露于铜绿微囊藻后，斑马鱼大脑中共有 82 个上调蛋白和 26 个下调蛋白。有趣的是，这些表达变化的蛋白与突触囊泡循环和萜类骨架生物合成途径有关，如 ACAT、STX1A 和 V-ATPase。这些结果一致表明，铜绿微囊藻的神经毒性严重破坏了斑马鱼神经系统中的神经递质传递以及大脑信息的存储和传输，使其更容易患神经疾病。我们的研究为蓝藻水华的神经毒性风险提供了新的视角。
