普罗色原蛙和齿蟾属（有尾目，齿蟾科）的精子形态比较。

Comparative sperm morphology of Proceratophrys and Odontophrynus (Anura, Odontophrynidae).

机构信息

Departamento de Biologia Estrutural e Funcional, Instituto de Biologia, Universidade Estadual de Campinas - Unicamp, 13083-863 Campinas, São Paulo, Brazil.

出版信息

Micron. 2019 Oct;125:102713. doi: 10.1016/j.micron.2019.102713. Epub 2019 Jul 25.

DOI:10.1016/j.micron.2019.102713

PMID:31419702

Abstract

The ultrastructure of the spermatozoa of seven species of Proceratophrys and one of Odontophrynus was analyzed in the present study. The sperm of Odontophrynus juquinha is similar to that of Odontophrynus cultripes, previously described, and to those of Proceratophrys branti, Proceratophrys bigibbosa, Proceratophrys affinis concavitympanum, Proceratophrys huntingtoni, Proceratophrys conformis moratoi, P. moratoi, and Proceratophrys palustris, in relation to the acrosomal vesicle and the subacrosomal cone that covers the anterior region of the nucleus in the sperm head. We observed transverse striations in the midpiece of the Odontophrynus juquinha sperm, a feature not observed in any Proceratophrys species. The sperm tail of all the studied species had a simple flagella and single axoneme associated with auxiliary structures (juxta-axonemal + undulating membrane + axial fiber). However, the juxta-axonemal fiber is thicker and the undulating membrane is shorter in Proceratophrys in comparison with Odontophrynus. In addition to differentiating these two genera, these features represent a possible autapomorphy in Proceratophrys.

摘要

本研究分析了 7 种原鼻蟾属和 1 种齿蟾属物种的精子超微结构。齿蟾属的 juquinha 精子与先前描述的齿蟾属 cultripes 以及原鼻蟾属的 branti、bigibbosa、affinis concavitympanum、huntingtoni、conformis moratoi、moratoi 和 palustris 的精子相似，在精子头部，顶体泡和覆盖核前区的亚顶体锥与精子的头部有关。我们观察到 Odontophrynus juquinha 精子中段的横向条纹，这在任何原鼻蟾属物种中都没有观察到。所有研究物种的精子尾部都有一个简单的鞭毛和一个与辅助结构（副轴丝+波动膜+轴纤维）相关的单一轴丝。然而，与 Odontophrynus 相比，原鼻蟾属的副轴丝纤维更厚，波动膜更短。除了区分这两个属之外，这些特征代表了原鼻蟾属的一个可能的自衍征。