CAS Key Laboratory of Chemistry of Northwestern Plant Resources and Key Laboratory for Natural Medicine of Gansu Province, Lanzhou Institute of Chemical Physics, Chinese Academy of Sciences, Lanzhou 730000, China; College of Chemical Engineering, Zhejiang University of Technology, Hangzhou 310014, China.
CAS Key Laboratory of Chemistry of Northwestern Plant Resources and Key Laboratory for Natural Medicine of Gansu Province, Lanzhou Institute of Chemical Physics, Chinese Academy of Sciences, Lanzhou 730000, China; University of Chinese Academy of Sciences, Chinese Academy of Sciences, Beijing 100039, China.
J Chromatogr A. 2020 Jan 4;1609:460446. doi: 10.1016/j.chroma.2019.460446. Epub 2019 Aug 9.
Two new copolymer-grafted silica stationary phases were prepared and employed in hydrophilic interaction chromatography (HILIC). 2-(Dimethylamino)ethyl methacrylate (DMAEMA) are copolymerized with itaconic acid (IA) and acrylic acid (AA) respectively, via thiol-ene click reaction on silica surface with deep eutectic solvents (DES) as new solvents. The obtained poly(DMAEMA-co-itaconic acid)-grafted silica (Sil-PDM-PIA) and poly(DMAEMA-co-acrylic acid)-grafted silica (Sil-PDM-PAA) were characterized by Fourier transform infrared spectroscopy, elemental analysis and solid-state C NMR spectra. Their hydrophilic interaction performances were evaluated by separating nucleosides, nucleobases, saccharides, and amino acids. Compared with previous reported poly(itaconic acid)-grafted silica (Sil-PIA) and poly(acrylic acid)-grafted silica (Sil-PAA) stationary phases, these two new copolymer-grafted silica performed higher selectivity and better separation for polar analytes in HILIC.
两种新型共聚硅胶固定相被制备并应用于亲水作用色谱（HILIC）中。通过巯基-烯点击反应，将 2-（二甲氨基）乙基甲基丙烯酸酯（DMAEMA）分别与衣康酸（IA）和丙烯酸（AA）共聚，在深共熔溶剂（DES）作为新型溶剂的硅胶表面上进行。得到的聚（DMAEMA-共-衣康酸）-接枝硅胶（Sil-PDM-PIA）和聚（DMAEMA-共-丙烯酸）-接枝硅胶（Sil-PDM-PAA）通过傅里叶变换红外光谱、元素分析和固态 C NMR 光谱进行了表征。通过分离核苷、碱基、糖和氨基酸来评估它们的亲水相互作用性能。与之前报道的聚（衣康酸）-接枝硅胶（Sil-PIA）和聚（丙烯酸）-接枝硅胶（Sil-PAA）固定相相比，这两种新型共聚硅胶在 HILIC 中对极性分析物表现出更高的选择性和更好的分离效果。
