Akinrinde A S, Adebiyi O E, Asekun A
Department of Veterinary Physiology and Biochemistry, Faculty of Veterinary Medicine, University of Ibadan, Ibadan, Nigeria.
J Complement Integr Med. 2019 Aug 15;17(1):/j/jcim.2019.17.issue-1/jcim-2019-0002/jcim-2019-0002.xml. doi: 10.1515/jcim-2019-0002.
Background Eucalyptus oil (EO), derived from Eucalyptus species, possesses vast remedial and healing properties, although its gut health-promoting properties have not been well investigated. In this study, we investigated the chemical composition of a commercial EO formulation and its potential role in protecting against aflatoxin B1 (AfB1)-induced gastrointestinal damage in rats. Methods Male Wistar rats were divided into six groups with eight rats each. Control rats were administered with the vehicle (1% Tween 80) for 14 days, while another group was exposed to two oral doses of AFB1 on days 12 and 14. Two other groups were pre-treated with oral doses of EO (50 and 100 mg/kg b.w.) for 14 consecutive days, along with two oral doses of AfB1 (5 mg/kg b.w.) on days 12 and 14. The remaining two groups were treated with EO alone at the two doses for 14 days. At the end of the experiment, blood samples, stomach and intestinal tissues were collected for measurement of oxidative stress and antioxidant parameters and light microscopic examination. Results Gas chromatography-mass spectrometry analysis revealed Eucalyptol (1, 8-cineole) as the main constituent (67.48%) of the oil. AfB1 administration induced oxidative and inflammatory disturbances, indicated by significantly (p<0.05) increased serum nitric oxide level and myeloperoxidase activity; increased tissue contents of hydrogen peroxide, malondialdehyde and protein carbonyls, accompanied with corresponding histological alterations. AfB1 also induced significant (p<0.05) reductions in glutathione peroxidase and superoxide dismutase (SOD) activities. Treatment with EO produced significant improvements in the biochemical parameters as well as the appearance of the gastric and intestinal mucosa. EO alone, at the two doses tested did not produce any significant changes in the parameters investigated. Conclusion The findings from this study showed that EO demonstrated protective activity against Aflatoxin-induced toxicity in stomach and intestinal tissues and may thus find application in treatment of gastrointestinal disorders.
背景 桉叶油（EO）源自桉属植物，具有多种治疗和愈合特性，但其促进肠道健康的特性尚未得到充分研究。在本研究中，我们调查了一种商业EO制剂的化学成分及其在预防黄曲霉毒素B1（AfB1）诱导的大鼠胃肠道损伤中的潜在作用。方法 将雄性Wistar大鼠分为六组，每组八只。对照组大鼠给予赋形剂（1%吐温80）14天，而另一组在第12天和第14天口服两次AfB1。另外两组连续14天口服EO（50和100mg/kg体重）进行预处理，同时在第12天和第14天口服两次AfB1（5mg/kg体重）。其余两组分别用两种剂量的EO单独治疗14天。实验结束时，采集血液样本、胃和肠道组织，用于测量氧化应激和抗氧化参数以及进行光学显微镜检查。结果 气相色谱-质谱分析显示桉叶油醇（1,8-桉叶素）是该油的主要成分（67.48%）。给予AfB1会引起氧化和炎症紊乱，表现为血清一氧化氮水平和髓过氧化物酶活性显著（p<0.05）升高；过氧化氢、丙二醛和蛋白质羰基的组织含量增加，并伴有相应的组织学改变。AfB1还会导致谷胱甘肽过氧化物酶和超氧化物歧化酶（SOD）活性显著（p<0.05）降低。用EO治疗可使生化参数以及胃和肠黏膜外观得到显著改善。单独使用两种测试剂量的EO在所研究的参数中未产生任何显著变化。结论 本研究结果表明，EO对黄曲霉毒素诱导的胃和肠道组织毒性具有保护作用，因此可能在胃肠道疾病的治疗中得到应用。
