Department of Molecular Biotechnology and Microbiology, University of Debrecen, Egyetem tér 1, Debrecen, H4032, Hungary.
Department of Genetics and Applied Microbiology, University of Debrecen, Egyetem tér 1, Debrecen, H4032, Hungary.
Microbiol Res. 2019 Oct;227:126298. doi: 10.1016/j.micres.2019.126298. Epub 2019 Jul 15.
An increasing number of infections originating from probiotic use are reported worldwide, with the majority of such cases caused by the yeast Saccharomyces 'boulardii', a subtype of S. cerevisiae. Reliably linking infectious cases to probiotic products requires unequivocal genotyping data, however, these techniques are often time-consuming and difficult to implement in routine diagnostics. This leads to a widespread lack of genetic data regarding the origin of Saccharomyces infections. We propose a quick and reliable PCR-based protocol for the identification of S. 'boulardii' based on a combined analysis of interdelta fingerprinting and microsatellite typing. By applying various typing methods and our proposed method to the clinical yeast collection of a Hungarian hospital we show that probiotic origin is common among clinical Saccharomyces, and that the new multiplex method enables rapid and unequivocal identification of probiotic yeast infections. This method can be applied for the identification of yeast infection sources, helping decisions on probiotic use.
越来越多的感染源自益生菌的使用在全球范围内被报道，其中大多数此类病例是由酵母 Saccharomyces 'boulardii'引起的，它是 S. cerevisiae 的一个亚型。然而，要将感染病例可靠地与益生菌产品联系起来，需要明确的基因分型数据，但这些技术往往耗时且难以在常规诊断中实施。这导致了关于 Saccharomyces 感染来源的遗传数据广泛缺乏。我们提出了一种基于 PCR 的快速可靠的方法，用于基于 interdelta 指纹分析和微卫星分型的 S. 'boulardii'的鉴定。通过将各种分型方法和我们提出的方法应用于匈牙利一家医院的临床酵母收集，我们表明益生菌来源在临床 Saccharomyces 中很常见，并且新的多重方法能够快速且明确地鉴定益生菌酵母感染。这种方法可用于鉴定酵母感染源，帮助做出益生菌使用的决策。
