Aksu İldeniz, Anlar Hatice Gül, Taner Gökçe, Bacanlı Merve, İritaş Servet, Tutkun Engin, Basaran Nursen
Department of Pharmaceutical Toxicology, Faculty of Pharmacy, Hacettepe University, 06100, Ankara, Turkey.
Department of Pharmaceutical Toxicology, Faculty of Pharmacy, Bülent Ecevit University, 67600, Zonguldak, Turkey.
Mutat Res Genet Toxicol Environ Mutagen. 2019 Jul;843:40-45. doi: 10.1016/j.mrgentox.2018.11.006. Epub 2018 Nov 19.
Welding technology is widely used in pressurized containers, thermal power plants, refineries, chemical facilities and steel structures. Welders are exposed to a number of hazardous compounds such as ultraviolet (UV) radiation, electromagnetic fields, toxic metals and polycyclic aromatic hydrocarbons (PAHs). In the present study, 48 welders and an equal number of control subjects were evaluated for DNA damage in the whole blood and isolated lymphocytes using the comet assay. The genotoxic damage in buccal epithelial cells of subjects was determined by micronucleus (MN) assay. Metal(loids) such as Cr, Mn, Ni, Cu, As, Cd and Pb levels in blood samples were evaluated by using Inductively Coupled Plasma-Mass Spectrometer (ICP-MS). Results of this study showed that DNA damage in blood, isolated lymphocytes, and buccal epithelial cells were significantly higher in workers compared to the controls. Also, these workers had remarkably higher blood Cr, Cu, Cd, Ni and Pb levels. These results showed that occupational exposure to welding fumes may cause genotoxic damage that can lead to important health problems in the workers. More extensive epidemiological studies should be performed that enable the assessment of health risk in welding industry.
焊接技术广泛应用于压力容器、火力发电厂、炼油厂、化工设施和钢结构中。焊工暴露于多种有害化合物中，如紫外线（UV）辐射、电磁场、有毒金属和多环芳烃（PAH）。在本研究中，使用彗星试验对48名焊工和同等数量的对照对象的全血和分离淋巴细胞中的DNA损伤进行了评估。通过微核（MN）试验确定受试者颊上皮细胞中的遗传毒性损伤。使用电感耦合等离子体质谱仪（ICP-MS）评估血样中铬、锰、镍、铜、砷、镉和铅等金属（类金属）的水平。本研究结果表明，与对照组相比，工人血液、分离淋巴细胞和颊上皮细胞中的DNA损伤明显更高。此外，这些工人血液中的铬、铜、镉、镍和铅水平显著更高。这些结果表明，职业接触焊接烟雾可能会导致遗传毒性损伤，从而使工人面临重要的健康问题。应进行更广泛的流行病学研究，以评估焊接行业的健康风险。
