ARF 蛋白的核质分配控制拟南芥中的生长素反应。
Nucleo-cytoplasmic Partitioning of ARF Proteins Controls Auxin Responses in Arabidopsis thaliana.
机构信息
Department of Biology, Washington University in St. Louis, St. Louis, MO 63130, USA.
Department of Biomedical Engineering, Washington University in St. Louis, St. Louis, MO 63130, USA.
出版信息
Mol Cell. 2019 Oct 3;76(1):177-190.e5. doi: 10.1016/j.molcel.2019.06.044. Epub 2019 Aug 14.
Abstract
The phytohormone auxin plays crucial roles in nearly every aspect of plant growth and development. The auxin response factor (ARF) transcription factor family regulates auxin-responsive gene expression and exhibits nuclear localization in regions of high auxin responsiveness. Here we show that the ARF7 and ARF19 proteins accumulate in micron-sized assemblies within the cytoplasm of tissues with attenuated auxin responsiveness. We found that the intrinsically disordered middle region and the folded PB1 interaction domain of ARFs drive protein assembly formation. Mutation of a single lysine within the PB1 domain abrogates cytoplasmic assemblies, promotes ARF nuclear localization, and results in an altered transcriptome and morphological defects. Our data suggest a model in which ARF nucleo-cytoplasmic partitioning regulates auxin responsiveness, providing a mechanism for cellular competence for auxin signaling.
摘要
植物激素生长素在植物生长和发育的几乎各个方面都起着至关重要的作用。生长素响应因子 (ARF) 转录因子家族调节生长素响应基因的表达，并在生长素响应性高的区域表现出核定位。在这里，我们表明 ARF7 和 ARF19 蛋白在生长素响应性减弱的组织的细胞质中积累在微米大小的组装体中。我们发现 ARF 的固有无序的中间区域和折叠的 PB1 相互作用域驱动蛋白组装的形成。PB1 结构域中单个赖氨酸的突变会破坏细胞质组装，促进 ARF 的核定位，并导致转录组改变和形态缺陷。我们的数据表明，ARF 的核质分配调节生长素的响应，为生长素信号的细胞能力提供了一种机制。
相似文献
1
Nucleo-cytoplasmic Partitioning of ARF Proteins Controls Auxin Responses in Arabidopsis thaliana.ARF 蛋白的核质分配控制拟南芥中的生长素反应。
Mol Cell. 2019 Oct 3;76(1):177-190.e5. doi: 10.1016/j.molcel.2019.06.044. Epub 2019 Aug 14.
2
A role for auxin response factor 19 in auxin and ethylene signaling in Arabidopsis.生长素响应因子19在拟南芥生长素和乙烯信号传导中的作用。
Plant Physiol. 2006 Mar;140(3):899-908. doi: 10.1104/pp.105.070987. Epub 2006 Feb 3.
3
Functional genomic analysis of the AUXIN RESPONSE FACTOR gene family members in Arabidopsis thaliana: unique and overlapping functions of ARF7 and ARF19.拟南芥生长素响应因子基因家族成员的功能基因组分析：ARF7和ARF19的独特与重叠功能
Plant Cell. 2005 Feb;17(2):444-63. doi: 10.1105/tpc.104.028316. Epub 2005 Jan 19.
4
The ARF7 and ARF19 Transcription Factors Positively Regulate in Arabidopsis Roots.ARF7 和 ARF19 转录因子正向调控拟南芥根中的 。
Plant Physiol. 2018 Sep;178(1):413-427. doi: 10.1104/pp.17.01713. Epub 2018 Jul 19.
5
The Arabidopsis transcription factor MYB77 modulates auxin signal transduction.拟南芥转录因子MYB77调控生长素信号转导。
Plant Cell. 2007 Aug;19(8):2440-53. doi: 10.1105/tpc.107.050963. Epub 2007 Aug 3.
6
Regulation of AUXIN RESPONSE FACTOR condensation and nucleo-cytoplasmic partitioning.生长素响应因子的凝聚和核质分配的调控。
Nat Commun. 2022 Jul 11;13(1):4015. doi: 10.1038/s41467-022-31628-2.
7
A secreted peptide acts on BIN2-mediated phosphorylation of ARFs to potentiate auxin response during lateral root development.一种分泌肽通过作用于 BIN2 介导的 ARF 磷酸化来增强侧根发育过程中的生长素反应。
Nat Cell Biol. 2014 Jan;16(1):66-76. doi: 10.1038/ncb2893. Epub 2013 Dec 22.
8
Defining a two-pronged structural model for PB1 (Phox/Bem1p) domain interaction in plant auxin responses.定义植物生长素应答中PB1（Phox/Bem1p）结构域相互作用的双管齐下结构模型。
J Biol Chem. 2015 May 15;290(20):12868-78. doi: 10.1074/jbc.M115.648253. Epub 2015 Apr 3.
9
Tissue-specific expression of stabilized SOLITARY-ROOT/IAA14 alters lateral root development in Arabidopsis.稳定化的单根/IAA14的组织特异性表达改变了拟南芥的侧根发育。
Plant J. 2005 Nov;44(3):382-95. doi: 10.1111/j.1365-313X.2005.02537.x.
10
Convergence of auxin and gibberellin signaling on the regulation of the GATA transcription factors GNC and GNL in Arabidopsis thaliana.生长素和赤霉素信号在调控拟南芥 GATA 转录因子 GNC 和 GNL 中的汇聚作用。
Proc Natl Acad Sci U S A. 2013 Aug 6;110(32):13192-7. doi: 10.1073/pnas.1304250110. Epub 2013 Jul 22.
引用本文的文献
1
A chaperonin BnaC01.CCT8 contributes to silique length and seed weight by affecting auxin and jasmonic acid signalling in Brassica napus.伴侣蛋白BnaC01.CCT8通过影响甘蓝型油菜中的生长素和茉莉酸信号传导，对角果长度和种子重量产生影响。
Plant Biotechnol J. 2025 Jun 18. doi: 10.1111/pbi.70184.
2
Genome-Wide Identification of / Negatively Regulate Root Growth in Soybean.大豆中负调控根系生长的全基因组鉴定
Int J Mol Sci. 2025 May 9;26(10):4547. doi: 10.3390/ijms26104547.
3
Active transport enables protein condensation in cells.主动运输使细胞中的蛋白质凝聚。
Sci Adv. 2025 May 23;11(21):eadv7875. doi: 10.1126/sciadv.adv7875.
4
Mechanisms of auxin action in plant growth and development.生长素在植物生长发育中的作用机制。
Nat Rev Mol Cell Biol. 2025 May 19. doi: 10.1038/s41580-025-00851-2.
5
Transcriptional Tuning: How Auxin Strikes Unique Chords in Gene Regulation.转录调控：生长素如何在基因调控中奏响独特乐章
Physiol Plant. 2025 May-Jun;177(3):e70229. doi: 10.1111/ppl.70229.
6
O-Fucosyltransferase SPINDLY attenuates auxin-induced fruit growth by inhibiting ARF6/8-coactivator mediator complex interaction in Arabidopsis.O-岩藻糖基转移酶SPINDLY通过抑制拟南芥中ARF6/8-共激活因子中介复合物的相互作用来减弱生长素诱导的果实生长。
Nat Commun. 2025 Apr 28;16(1):3965. doi: 10.1038/s41467-025-59095-5.
7
Unresolved roles of Aux/IAA proteins in auxin responses.生长素/吲哚乙酸（Aux/IAA）蛋白在生长素应答中尚未明确的作用。
Physiol Plant. 2025 Mar-Apr;177(2):e70221. doi: 10.1111/ppl.70221.
8
Comparative mutant analyses reveal a novel mechanism of ARF regulation in land plants.比较突变体分析揭示了陆地植物中ARF调控的新机制。
Nat Plants. 2025 Apr;11(4):821-835. doi: 10.1038/s41477-025-01973-3. Epub 2025 Apr 11.
9
An improved toolkit of gateway- and gibson assembly-compatible vectors for protoplast transfection and agrobacterium-mediated plant transformation.一种经过改进的用于原生质体转染和农杆菌介导的植物转化的、与Gateway和吉布森组装兼容的载体工具包。
BMC Res Notes. 2025 Apr 8;18(1):149. doi: 10.1186/s13104-025-07231-1.
10
Behind phyllotaxis, within the meristem: a REM-ARF complex shapes inflorescence in Arabidopsis thaliana.在拟南芥叶序背后，分生组织内部：一个REM-ARF复合体塑造花序。
Plant J. 2025 Mar;121(5):e70041. doi: 10.1111/tpj.70041.
本文引用的文献
1
Phase Separation of Intrinsically Disordered Proteins.内在无序蛋白质的相分离
Methods Enzymol. 2018;611:1-30. doi: 10.1016/bs.mie.2018.09.035. Epub 2018 Oct 31.
2
Transcription Factors Activate Genes through the Phase-Separation Capacity of Their Activation Domains.转录因子通过其激活结构域的相分离能力激活基因。
Cell. 2018 Dec 13;175(7):1842-1855.e16. doi: 10.1016/j.cell.2018.10.042. Epub 2018 Nov 15.
3
A Molecular Grammar Governing the Driving Forces for Phase Separation of Prion-like RNA Binding Proteins.一种分子语法，用于控制朊病毒样 RNA 结合蛋白相分离的驱动力。
Cell. 2018 Jul 26;174(3):688-699.e16. doi: 10.1016/j.cell.2018.06.006. Epub 2018 Jun 28.
4
Mediator and RNA polymerase II clusters associate in transcription-dependent condensates.中介体和 RNA 聚合酶 II 簇在转录依赖性凝聚物中聚集。
Science. 2018 Jul 27;361(6400):412-415. doi: 10.1126/science.aar4199. Epub 2018 Jun 21.
5
Coactivator condensation at super-enhancers links phase separation and gene control.共激活因子在超级增强子上的凝聚将相分离和基因调控联系起来。
Science. 2018 Jul 27;361(6400). doi: 10.1126/science.aar3958. Epub 2018 Jun 21.
6
Imaging dynamic and selective low-complexity domain interactions that control gene transcription.成像动态和选择性的低复杂度结构域相互作用，从而控制基因转录。
Science. 2018 Jul 27;361(6400). doi: 10.1126/science.aar2555. Epub 2018 Jun 21.
7
Different Material States of Pub1 Condensates Define Distinct Modes of Stress Adaptation and Recovery.Pub1 凝聚物的不同物质状态定义了不同的应激适应和恢复模式。
Cell Rep. 2018 Jun 12;23(11):3327-3339. doi: 10.1016/j.celrep.2018.05.041.
8
Phase-separation mechanism for C-terminal hyperphosphorylation of RNA polymerase II.RNA 聚合酶 II C 端高度磷酸化的相分离机制。
Nature. 2018 Jun;558(7709):318-323. doi: 10.1038/s41586-018-0174-3. Epub 2018 May 30.
9
Ubiquitin Modulates Liquid-Liquid Phase Separation of UBQLN2 via Disruption of Multivalent Interactions.泛素通过破坏多价相互作用调节 UBQLN2 的液-液相分离。
Mol Cell. 2018 Mar 15;69(6):965-978.e6. doi: 10.1016/j.molcel.2018.02.004. Epub 2018 Mar 8.
10
Polyubiquitin chain-induced p62 phase separation drives autophagic cargo segregation.多泛素链诱导 p62 相分离驱动自噬货物的隔离。
Cell Res. 2018 Apr;28(4):405-415. doi: 10.1038/s41422-018-0017-7. Epub 2018 Mar 5.
Zotero 插件