Zhu Yi-Fang, Guo Yuan-Biao, Zhang Han-Yu, Yang Peng, Wei Dan-Feng, Zhang Tong-Tong, Pan Bi-Ran, Liu Lei
Clinical Laboratory, Sichuan Provincial Orthopedic Hospital, Chengdu, Sichuan 610041, P.R. China.
Medical Research Center, The Second Affiliated Clinical College of Chongqing Medical University, The Third People's Hospital of Chengdu, Chengdu, Sichuan 610031, P.R. China.
Oncol Lett. 2019 Aug;18(2):1863-1871. doi: 10.3892/ol.2019.10482. Epub 2019 Jun 14.
Contactin 3 (CNTN3) is a member of the contactin family that is primarily expressed in the nervous system. However, to the best of our knowledge, expression of contactin and its role in the development and progression of brain tumours has not been studied. Although glioblastoma multiforme (GBM) is the most common malignant brain tumour, advances in therapeutic options for patients with GBM have been modest due to an incomplete understanding of the molecular mechanisms underlying development and progression. The aim of the present study was to examine the correlation between and its associated genes and the clinical outcome in patients with GBM. and the expression levels of associated genes were analysed in GBM datasets obtained from the SAGE Anatomical viewer website, Gene Expression Omnibus, Oncomine and The Cancer Genome Atlas. was significantly downregulated in patients with GBM. Subsequently, the expression of was further validated using immunohistochemistry in a cohort of GBM specimens. The immunohistochemistry results were consistent with the analyses. Kaplan-Meier analysis indicated that patients with lower expression levels of had a significantly shorter overall survival (OS) time compared with patients with higher levels of expression. Univariate and multivariate Cox regression analyses demonstrated that expression was an independent prognostic indicator in patients with GBM. Furthermore, gene set enrichment analysis revealed that was associated with the receptor tyrosine-protein kinase (ErbB) signalling pathway. In the ErbB signalling pathway, epidermal growth factor receptor (EGFR) was negatively correlated with . Taken together, these data suggest that lower expression levels of may be an independent biomarker that predicts poor OS time in patients with GBM, and that EGFR expression in the ErbB pathway may be associated with expression.
接触蛋白3（CNTN3）是接触蛋白家族的成员，主要在神经系统中表达。然而，据我们所知，尚未对接触蛋白在脑肿瘤发生和发展中的表达及其作用进行研究。虽然多形性胶质母细胞瘤（GBM）是最常见的恶性脑肿瘤，但由于对其发生和发展的分子机制了解不全面，GBM患者的治疗选择进展有限。本研究的目的是探讨CNTN3及其相关基因与GBM患者临床结局之间的相关性。在从SAGE解剖学查看器网站、基因表达综合数据库、Oncomine和癌症基因组图谱获得的GBM数据集中分析了CNTN3及其相关基因的表达水平。GBM患者中CNTN3显著下调。随后，在一组GBM标本中使用免疫组织化学进一步验证了CNTN3的表达。免疫组织化学结果与基因表达分析结果一致。Kaplan-Meier分析表明，与CNTN3表达水平较高的患者相比，CNTN3表达水平较低的患者总生存期（OS）明显较短。单因素和多因素Cox回归分析表明，CNTN3表达是GBM患者的独立预后指标。此外，基因集富集分析显示CNTN3与受体酪氨酸蛋白激酶（ErbB）信号通路相关。在ErbB信号通路中，表皮生长因子受体（EGFR）与CNTN3呈负相关。综上所述，这些数据表明，较低的CNTN3表达水平可能是预测GBM患者OS时间较短的独立生物标志物，并且ErbB通路中的EGFR表达可能与CNTN3表达相关。
