免疫层析法检测尿中的α-微管蛋白用于诊断肾损伤。
Immunochromatographic assay to detect α-tubulin in urine for the diagnosis of kidney injury.
机构信息
Companion Diagnostics and Medical Technology Research Group, Daegu Gyeongbuk Institute of Science and Technology (DGIST), Daegu, Korea.
Department of Internal Medicine, Kyungpook National University Hospital, Daegu, Korea.
出版信息
J Clin Lab Anal. 2020 Jan;34(1):e23015. doi: 10.1002/jcla.23015. Epub 2019 Aug 19.
BACKGROUNDS
Shortening of primary cilia in kidney epithelial cells is associated with kidney injury and involved with the induced level of α-tubulin in urine. Therefore, rapid detection and quantification of α-tubulin in the urine samples could be used to the preliminary diagnosis of kidney injury.
METHODS
Cellulose-based nanobeads modified with α-tubulin were used for the detection probe of competitive immunochromatographic (IC) assay. The concentration of α-tubulin in the urine samples was determined by IC assay and compared with the amount determined by Western blotting analysis.
RESULTS
The relationship between α-tubulin concentration and the colorimetric intensity resulted from IC assay was determined by logistic regression, and the correlation coefficient (R ) was 0.9948. When compared to the amount determined by Western blotting analysis, there was a linear relationship between the α-tubulin concentrations measured by the two methods and the R value was 0.823.
CONCLUSIONS
This method is simple, rapid, and adequately sensitive to detect α-tubulin in patient urine samples, which could be used for the clinical diagnosis of kidney injury.
背景
肾脏上皮细胞中初级纤毛的缩短与肾脏损伤有关，并与尿液中α-微管蛋白的诱导水平有关。因此，快速检测和定量尿液样本中的α-微管蛋白可用于肾脏损伤的初步诊断。
方法
用α-微管蛋白修饰的基于纤维素的纳米珠作为竞争免疫层析（IC）测定的检测探针。通过 IC 测定法确定尿液样本中α-微管蛋白的浓度，并与 Western 印迹分析确定的量进行比较。
结果
通过逻辑回归确定了 IC 测定法中α-微管蛋白浓度与比色强度之间的关系，相关系数（R）为 0.9948。与 Western 印迹分析确定的量相比，两种方法测量的α-微管蛋白浓度之间存在线性关系，R 值为 0.823。
结论
该方法简单、快速，足以灵敏地检测患者尿液样本中的α-微管蛋白，可用于肾脏损伤的临床诊断。
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