Department of Spine Surgery and Institute for Orthopaedic Research, The 2nd Clinical Medical College (Shenzhen People's Hospital) of Jinan University, Shenzhen, 518020, China.
Integrated Chinese and Western Medicine Postdoctoral Research Station, Jinan University, Guangzhou, 510632, China.
Small. 2019 Oct;15(41):e1901560. doi: 10.1002/smll.201901560. Epub 2019 Aug 18.
Tissue-engineered hydrogels have received extensive attention as their mechanical properties, chemical compositions, and biological signals can be dynamically modified for mimicking extracellular matrices (ECM). Herein, the synthesis of novel double network (DN) hydrogels with tunable mechanical properties using combinatorial screening methods is reported. Furthermore, nanoengineered (NE) hydrogels are constructed by addition of ultrathin 2D black phosphorus (BP) nanosheets to the DN hydrogels with multiple functions for mimicking the ECM microenvironment to induce tissue regeneration. Notably, it is found that the BP nanosheets exhibit intrinsic properties for induced CaP crystal particle formation and therefore improve the mineralization ability of NE hydrogels. Finally, in vitro and in vivo data demonstrate that the BP nanosheets, mineralized CaP crystal nanoparticles, and excellent mechanical properties provide a favorable ECM microenvironment to mediate greater osteogenic cell differentiation and bone regeneration. Consequently, the combination of bioactive chemical materials and excellent mechanical stimuli of NE hydrogels inspire novel engineering strategies for bone-tissue regeneration.
组织工程水凝胶因其机械性能、化学组成和生物信号可以动态调节，从而模拟细胞外基质（ECM），受到了广泛关注。本文报道了采用组合筛选方法合成具有可调机械性能的新型双网络（DN）水凝胶。此外，通过向 DN 水凝胶中加入超薄二维黑磷（BP）纳米片，构建了纳米工程（NE）水凝胶，具有多种功能，可模拟 ECM 微环境，诱导组织再生。值得注意的是，发现 BP 纳米片具有内在特性，可诱导 CaP 晶体颗粒形成，从而提高 NE 水凝胶的矿化能力。最后，体外和体内数据表明，BP 纳米片、矿化 CaP 晶体纳米颗粒和优异的机械性能为成骨细胞分化和骨再生提供了有利的 ECM 微环境。因此，生物活性化学材料与 NE 水凝胶的优异机械刺激相结合，为骨组织再生提供了新的工程策略。
