Department of Endodontics, Guangdong Provincial High-level Clinical Key Specialty, Guangdong Province Engineering Research Center of Oral Disease Diagnosis and Treatment, Peking University Shenzhen Hospital, Shenzhen, Guangdong 518036, PR China.
Guangdong Provincial High-level Clinical Key Specialty, Guangdong Province Engineering Research Center of Oral Disease Diagnosis and Treatment, Peking University Shenzhen Hospital, Shenzhen, Guangdong 518036, PR China.
Colloids Surf B Biointerfaces. 2024 Jul;239:113971. doi: 10.1016/j.colsurfb.2024.113971. Epub 2024 May 14.
The optimal material for repairing skull defects should exhibit outstanding biocompatibility and mechanical properties. Specifically, hydrogel scaffolds that emulate the microenvironment of the native bone extracellular matrix play a vital role in promoting osteoblast adhesion, proliferation, and differentiation, thereby yielding superior outcomes in skull reconstruction. In this study, a composite network hydrogel comprising sodium alginate (SA), epigallocatechin gallate (EGCG), and zinc ions (Zn) was developed to establish an ideal osteogenic microenvironment for bone regeneration. Initially, physical entanglement and hydrogen bonding between SA and EGCG resulted in the formation of a primary network hydrogel known as SA-EGCG. Subsequently, the inclusion of Zn facilitated the creation of a composite network hydrogels named SA-EGCG-Zn via dynamic coordination bonds with SA and EGCG. The engineered SA-EGCG2 %-Zn hydrogels offered an environment mimicking the native extracellular matrix (ECM). Moreover, the sustained release of Zn from the hydrogel effectively enhanced cell adhesion, promoted proliferation, and stimulated osteoblast differentiation. In vitro experiments have shown that SA-EGCG2 %-Zn hydrogels greatly enhance the attachment and growth of osteoblast precursor cells (MC3T3-E1), while also increasing the expression of genes related to osteogenesis in these cells. Additionally, in vivo studies have confirmed that SA-EGCG2 %-Zn hydrogels promote new bone formation and accelerate the regeneration of bone in situ, indicating promising applications in the realm of bone tissue engineering.
用于修复颅骨缺损的最佳材料应具有出色的生物相容性和机械性能。具体来说，模仿天然骨细胞外基质微环境的水凝胶支架在促进成骨细胞黏附、增殖和分化方面起着至关重要的作用，从而在颅骨重建中取得更好的效果。在这项研究中，开发了一种由海藻酸钠 (SA)、表没食子儿茶素没食子酸酯 (EGCG) 和锌离子 (Zn) 组成的复合网络水凝胶，以建立用于骨再生的理想成骨微环境。首先，SA 和 EGCG 之间的物理缠结和氢键作用形成了一种称为 SA-EGCG 的初级网络水凝胶。随后，通过与 SA 和 EGCG 的动态配位键，加入 Zn 促进了复合网络水凝胶 SA-EGCG-Zn 的形成。工程化的 SA-EGCG2%-Zn 水凝胶提供了一种模仿天然细胞外基质 (ECM) 的环境。此外，水凝胶中 Zn 的持续释放有效地增强了细胞黏附、促进了增殖，并刺激了成骨细胞分化。体外实验表明，SA-EGCG2%-Zn 水凝胶极大地增强了成骨前体细胞 (MC3T3-E1) 的附着和生长，同时还增加了这些细胞中成骨相关基因的表达。此外，体内研究证实，SA-EGCG2%-Zn 水凝胶促进新骨形成并加速原位骨再生，表明其在骨组织工程领域具有广阔的应用前景。
