Department of Anesthesiology , the Second Affiliated Hospital and Yuying Children's Hospital of Wenzhou Medical University , Wenzhou 325000 , China.
Chem Res Toxicol. 2019 Sep 16;32(9):1772-1779. doi: 10.1021/acs.chemrestox.9b00123. Epub 2019 Sep 3.
Polybrominated diphenyl ethers (PBDEs) are a group of flame retardants with two or more bromines attached. They are endocrine disruptors. PBDEs photodegrade into 4-bromodiphenyl ether (BDE3). Whether BDE3 impairs adrenal cortical cell function during postnatal development still remains unknown. The aim of the current study was to investigate the influence of BDE3 on adrenal cortical cell function. Sprague-Dawley rats (35 days of age, male) were orally administered with BDE3 (0, 50, 100, and 200 mg/kg/day body weight) for 21 days. BDE3 significantly increased serum aldosterone and corticosterone levels at 200 mg/kg without affecting adrenocorticotropic hormone level. Further study showed that BDE3 up-regulated at 100 and 200 mg/kg and , , , , and mRNA levels in the 200 mg/kg group. BDE3 also decreased the phosphorylation of AMP-activated protein kinase (AMPK) at 200 mg/kg and increased PGC-1α and phosphorylated cyclic AMP-responsive element-binding protein (CREB)/CREB at 200 mg/kg. Taken together, these findings demonstrate that BDE3 stimulates adrenal cell function likely through decreasing phosphorylation of AMPK and increasing phosphorylation of CREB.
多溴联苯醚（PBDEs）是一组含有两个或多个溴原子的阻燃剂。它们是内分泌干扰物。PBDEs 光降解为 4-溴二苯醚（BDE3）。BDE3 在出生后发育过程中是否会损害肾上腺皮质细胞功能仍不清楚。本研究旨在探讨 BDE3 对肾上腺皮质细胞功能的影响。雄性 Sprague-Dawley 大鼠（35 日龄）经口给予 BDE3（0、50、100 和 200mg/kg/天体重）21 天。BDE3 在 200mg/kg 时显著增加血清醛固酮和皮质酮水平，而不影响促肾上腺皮质激素水平。进一步的研究表明，BDE3 在 100 和 200mg/kg 时上调 ，在 200mg/kg 组上调 、 、 、 、 和 mRNA 水平。BDE3 还降低了 200mg/kg 时 AMP 激活的蛋白激酶（AMPK）的磷酸化，并增加了 PGC-1α 和磷酸化的环腺苷酸反应元件结合蛋白（CREB）/CREB 在 200mg/kg 时的磷酸化。综上所述，这些发现表明 BDE3 通过降低 AMPK 的磷酸化和增加 CREB 的磷酸化来刺激肾上腺细胞功能。
