Gaur Bharti, Saha Siddhartha, Iuliano A Danielle, Rai Sanjay K, Krishnan Anand, Jain Seema, Whitaker Brett, Winchell Jonas, Lal Renu B, Broor Shobha
Department of Microbiology, All Institute of Medical Sciences, New Delhi, India.
Influenza Division, Centers for Disease Control and Prevention, U.S Embassy, New Delhi, India.
Indian J Med Microbiol. 2019 Jan-Mar;37(1):105-108. doi: 10.4103/ijmm.IJMM_18_146.
Historical specimens collected from hospitalized children were tested for the following 13 viruses: influenza A and B; respiratory syncytial virus (RSV); parainfluenza viruses 1-3; human metapneumovirus; rhinovirus; coronaviruses 229E, OC43, NL63 and HKU1 and Adenovirus using monoplex real-time reverse transcriptase polymerase chain reaction (rRT-PCR). They were retested using TaqMan Array Card (TAC), a micro-fluidic system, capable of simultaneous multi-pathogen testing, to evaluate its sensitivity and specificity against monoplex rRT-PCR. TAC showed high sensitivity (71%-100%) and specificity (98%-100%) for these viruses in comparison to monoplex rRT-PCR. Multi-specimen detection with high sensitivity and specificity makes TAC a potentially useful tool for both surveillance and outbreak investigations.
对从住院儿童身上采集的历史样本进行了以下13种病毒的检测：甲型和乙型流感病毒；呼吸道合胞病毒（RSV）；副流感病毒1-3型；人偏肺病毒；鼻病毒；冠状病毒229E、OC43、NL63和HKU1以及腺病毒，采用单重实时逆转录聚合酶链反应（rRT-PCR）。使用TaqMan Array Card（TAC）（一种能够同时进行多病原体检测的微流控系统）对它们进行重新检测，以评估其相对于单重rRT-PCR的敏感性和特异性。与单重rRT-PCR相比，TAC对这些病毒显示出高敏感性（71%-100%）和特异性（98%-100%）。具有高敏感性和特异性的多样本检测使TAC成为监测和疫情调查的潜在有用工具。
