Greve C, Opsahl W, Reiser K, Abbott U, Kenney C, Benson D, Rucker R
Department of Nutrition (College of Agricultural and Environmental Sciences), University of California, Davis 95616.
Biochim Biophys Acta. 1988 Nov 17;967(2):275-83. doi: 10.1016/0304-4165(88)90020-7.
The amounts of lysine-derived crosslinks in collagens from tendon, cartilage, intervertebral disc, and bone and changes in the composition of sternal cartilage glycosaminoglycans were estimated in two lines of chickens, a control-isogenic line and a line that develops scoliosis. In the scoliotic line, scoliosis first appears at 3-4 weeks and progressively increases in severity and incidence so that 90% of the birds express the lesion by week 10. We have reported previously that cartilage, tendon, and bone collagens from scoliotic birds are more soluble than corresponding collagens from normal birds. Herein, collagen crosslinking and altered proteoglycan metabolism are examined as possible mechanisms for the differences in collagen solubility. At 1 week of age there were fewer reducible crosslinking amino acids (hydroxylysinonorleucine, dihydroxylysinonorleucine, and lysinonorleucine) in collagens from sternal cartilage and tendon in the scoliotic line than in the isogenic line. However, by week 3 and at weeks 5 or 7 values were similar in both groups. The amounts of hydroxypyridinium in vertebral bone and intervertebral disc collagen were also similar in both groups of birds. Consequently, differences in collagen crosslinking do not appear to be a persistent developmental defect underlying the expression of scoliosis in the model. However, differences were observed in cartilage proteoglycans and glycosaminoglycans from the scoliotic line that were not present in cartilage from the isogenic line. The average molecular weight of the uronide-containing glycosaminoglycans was 30% less in the scoliotic line than in the isogenic line, i.e., 12,000 compared to 18,000. The size distribution of cartilage proteoglycans from the scoliotic line also differed from that of proteoglycans from the isogenic line.(ABSTRACT TRUNCATED AT 250 WORDS)
在两组鸡中，即对照同基因系和发生脊柱侧弯的品系，对来自肌腱、软骨、椎间盘和骨骼的胶原蛋白中赖氨酸衍生交联的量以及胸骨软骨糖胺聚糖组成的变化进行了估计。在脊柱侧弯品系中，脊柱侧弯首先在3 - 4周出现，严重程度和发生率逐渐增加，到第10周时90%的鸡出现该病变。我们之前报道过，脊柱侧弯鸡的软骨、肌腱和骨骼胶原蛋白比正常鸡的相应胶原蛋白更易溶解。在此，研究胶原蛋白交联和蛋白聚糖代谢改变作为胶原蛋白溶解性差异的可能机制。在1周龄时，脊柱侧弯品系胸骨软骨和肌腱胶原蛋白中可还原交联氨基酸（羟赖氨酸正亮氨酸、二羟赖氨酸正亮氨酸和赖氨酸正亮氨酸）的数量比同基因系少。然而，到第3周以及第5周或第7周时，两组的值相似。两组鸡的椎骨和椎间盘胶原蛋白中的羟基吡啶鎓含量也相似。因此，胶原蛋白交联的差异似乎不是该模型中脊柱侧弯表达的持续性发育缺陷。然而，在脊柱侧弯品系的软骨蛋白聚糖和糖胺聚糖中观察到了同基因系软骨中不存在的差异。脊柱侧弯品系中含糖醛酸的糖胺聚糖的平均分子量比同基因系低30%，即分别为12,000和18,000。脊柱侧弯品系软骨蛋白聚糖的大小分布也与同基因系蛋白聚糖不同。（摘要截断于250字）
