Beard H K, Roberts S, O'Brien J P
J Bone Joint Surg Br. 1981;63B(4):529-34. doi: 10.1302/0301-620X.63B4.6170646.
Specific antisera to collagen Types I, II and III and proteoglycan were used to investigate the distributions of these molecules in normal human intervertebral discs. Immunofluorescent staining indicated the presence of small amounts of Type III collagen located pericellularly in normal adult intervertebral discs. This finding had not been demonstrated previously by other methods. Similar specimens of intervertebral discs from 17 patients with scoliosis of varying aetiologies were examined, but no evidence was obtained for primary connective tissue defects. Secondary changes, especially marked vascularisation of the inner annulus, were apparent in a number of scoliotic discs, and some of these showed enhanced staining for collagen Type I and proteoglycan, and intercellular matrix staining for Type III collagen.
使用针对I型、II型和III型胶原蛋白以及蛋白聚糖的特异性抗血清来研究这些分子在正常人类椎间盘中的分布。免疫荧光染色表明，在正常成人椎间盘中，少量III型胶原蛋白存在于细胞周围。这一发现以前未被其他方法证实。对17例病因各异的脊柱侧弯患者的类似椎间盘标本进行了检查，但未获得原发性结缔组织缺陷的证据。在一些脊柱侧弯的椎间盘中明显出现了继发性变化，尤其是内环明显血管化，其中一些显示I型胶原蛋白和蛋白聚糖染色增强，以及III型胶原蛋白的细胞间基质染色增强。
