Molecular & Cell Biology, College of Public Health, Medical & Veterinary Sciences, James Cook University. Townsville, Australia.
One Health Research Group, Melbourne Veterinary School, Faculty of Veterinary and Agricultural Sciences, University of Melbourne, Werribee, Victoria 3030, Australia.
J Microbiol Methods. 2019 Oct;165:105688. doi: 10.1016/j.mimet.2019.105688. Epub 2019 Aug 16.
The fungus Batrachochytrium dendrobatidis is causing global amphibian declines. Here we describe a simple, rapid and inexpensive methylene blue staining protocol to determine B. dendrobatidis viability, regardless of life-stage. The viability of cells in suspension or adherent monolayers can be determined using either manual microscopy counting or colorimetric assay.
蛙壶菌正在导致全球两栖动物的减少。在这里，我们描述了一种简单、快速且廉价的亚甲基蓝染色方案，用于确定蛙壶菌的活力，无论其生活阶段如何。使用手动显微镜计数或比色测定法均可确定悬浮或贴壁单层细胞的活力。
