埃及糖尿病肾病患者尿足细胞相关mRNA谱
Urinary podocyte-associated mRNA profile in Egyptian patients with diabetic nephropathy.
作者信息
Fayed Ahmed, Rahman Tohamy Iman Abdul, Kahla Hala, Elsayed Naglaa M, El Ansary Mervat, Saadi Gamal
机构信息
Nephrology unit, Internal Medicine Department, School of Medicine, Cairo University, Egypt.
Endocrinology unit, Internal Medicine Department, School of Medicine, Cairo University, Egypt.
出版信息
Diabetes Metab Syndr. 2019 Sep-Oct;13(5):2849-2854. doi: 10.1016/j.dsx.2019.07.048. Epub 2019 Jul 29.
INTRODUCTION
Podocyte injury and subsequent excretion in urine play a crucial role in the pathogenesis and progression of diabetic nephropathy (DN). Quantification of messenger RNA expression in urinary sediment by real-time PCR is emerging as a noninvasive method of screening DN-associated biomarkers. We aimed to study the expression of podocyte-associated genes in urinary sediment and their relation to disease severity in type 2 diabetic Egyptian patients with diabetic nephropathy.
METHOD
ology: Sixty patients with type 2 diabetes mellitus were recruited in addition to twenty non diabetic healthy volunteers. Relative mRNA abundance of nephrin, podocalyxin, and podocin were quantified, and correlations between target mRNAs and clinical parameters were examined.
RESULTS
The urinary mRNA levels of all genes studied were significantly higher in diabetics compared with controls (p < 0.001), and mRNA levels increased with DN progression. Urinary mRNA levels of all target genes positively correlated with both UAE and HbA. The expression of nephrin, podocalyxin, and podocin mRNA correlated with serum creatinine {(r = 0.397, p value = 0.002), (r = 0.431, p value = 0.001), (r = 0.433, p value = 0.001) respectively}.
CONCLUSION
The urinary mRNA profiles of nephrin, podocalyxin, and podocin were found to increase with the progression of DN, which suggested that quantification of podocyte-associated molecules will be useful biomarkers of DN.
引言
足细胞损伤及随后的尿液排泄在糖尿病肾病（DN）的发病机制和进展中起关键作用。通过实时聚合酶链反应（PCR）对尿沉渣中的信使核糖核酸（mRNA）表达进行定量分析，正成为一种筛查DN相关生物标志物的非侵入性方法。我们旨在研究2型糖尿病埃及DN患者尿沉渣中足细胞相关基因的表达及其与疾病严重程度的关系。
方法
招募了60例2型糖尿病患者以及20名非糖尿病健康志愿者。对nephrin、足细胞表面蛋白和足动蛋白的相对mRNA丰度进行定量，并检测目标mRNA与临床参数之间的相关性。
结果
与对照组相比，糖尿病患者中所有研究基因的尿mRNA水平均显著升高（p < 0.001），且mRNA水平随DN进展而升高。所有目标基因的尿mRNA水平与尿白蛋白排泄率（UAE）和糖化血红蛋白（HbA）均呈正相关。nephrin、足细胞表面蛋白和足动蛋白mRNA的表达与血清肌酐相关{（r = 0.397，p值 = 0.002），（r = 0.431，p值 = 0.001），（r = 0.433，p值 = 0.001）}。
结论
发现nephrin、足细胞表面蛋白和足动蛋白的尿mRNA谱随DN进展而增加，这表明对足细胞相关分子进行定量分析将成为DN有用的生物标志物。
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