Jeelani Humira, Ganie Mohd Ashraf, Masood Akbar, Amin Shajrul, Kawa Iram Ashaq, Fatima Qudsia, Manzoor Saika, Parvez Tabasum, Naikoo Niyaz Ahmad, Rashid Fouzia
Department of Biochemistry, University of Kashmir, Srinagar, Jammu and Kashmir, India; Department of Clinical Biochemistry, University of Kashmir, Srinagar, Jammu and Kashmir, India.
Department of Endocrinology, Sher-I-Kashmir Institute of Medical Sciences, Srinagar, Jammu and Kashmir, India.
Diabetes Metab Syndr. 2019 Sep-Oct;13(5):2907-2915. doi: 10.1016/j.dsx.2019.08.001. Epub 2019 Aug 2.
Polycystic Ovary Syndrome (PCOS) is the most common female endocrinopathy among premenopausal women associated with hyperandrogenism, obesity, dyslipidemia, insulin resistance and inflammation. Oxidative stress is an important component of cardio-metabolic risk seen in PCOS.
A total of 95 women with PCOS and 95 healthy controls were included in this observational study. Serum PON1 activity and stress markers were measured by spectrophotometric methods. Circulating TF level was measured by ELISA.
We found decreased PON1 activity and increased TF levels in women with PCOS compared to healthy controls. Fasting insulin, HOMA-IR, testosterone, LDL-C, MDA, PC and SOD activity were significantly increased whereas FGIR, QUICKI, HDLC, CAT and TAC were significantly decreased in PCOS women than controls. We observed a positive association of PON1 activity with FGIR, QUICKI, HDL-C and TAC, and its negative association was observed with LH, testosterone, fasting insulin and HOMA-IR in PCOS women. We further observed a positive association of TF with waist, waist to hip ratio, BMI, glucose 1hr, cholesterol, LDL-C, SGPT, uric acid and SOD activity in PCOS women.
Decreased PON1 activity and raised circulating TF levels are respective indicators of pro-inflammatory and procoagulant status in PCOS women. The imbalanced oxidant/antioxidant status further supports the evidences that PCOS is an oxidant state. Further, the association of PON1 activity and TF levels with the clinical, laboratory findings and stress marker levels suggest that these factors taken together are involved in aggravating the pro-inflammatory status in PCOS women.
多囊卵巢综合征（PCOS）是绝经前女性中最常见的女性内分泌病，与高雄激素血症、肥胖、血脂异常、胰岛素抵抗和炎症相关。氧化应激是PCOS中心血管代谢风险的重要组成部分。
本观察性研究共纳入95例PCOS女性和95例健康对照。采用分光光度法测定血清对氧磷酶1（PON1）活性和应激标志物。采用酶联免疫吸附测定法（ELISA）测定循环组织因子（TF）水平。
我们发现，与健康对照相比，PCOS女性的PON1活性降低，TF水平升高。PCOS女性的空腹胰岛素、稳态模型评估胰岛素抵抗（HOMA-IR）、睾酮、低密度脂蛋白胆固醇（LDL-C）、丙二醛（MDA）、血浆铜蓝蛋白（PC）和超氧化物歧化酶（SOD）活性显著升高，而空腹血糖胰岛素抵抗指数（FGIR）、定量胰岛素敏感性检查指数（QUICKI）、高密度脂蛋白胆固醇（HDL-C）、过氧化氢酶（CAT）和总抗氧化能力（TAC）显著降低。我们观察到，在PCOS女性中，PON1活性与FGIR、QUICKI、HDL-C和TAC呈正相关，与促黄体生成素（LH）、睾酮、空腹胰岛素和HOMA-IR呈负相关。我们进一步观察到，在PCOS女性中，TF与腰围、腰臀比、体重指数（BMI）、1小时血糖、胆固醇、LDL-C、谷丙转氨酶（SGPT）、尿酸和SOD活性呈正相关。
PON1活性降低和循环TF水平升高分别是PCOS女性促炎和促凝血状态的指标。氧化/抗氧化状态失衡进一步支持了PCOS是一种氧化状态的证据。此外，PON1活性和TF水平与临床、实验室检查结果及应激标志物水平的关联表明，这些因素共同作用加剧了PCOS女性的促炎状态。
