Chełchowska Magdalena, Jurczewska Justyna, Gajewska Joanna, Mazur Joanna, Szostak-Węgierek Dorota, Rudnicka Ewa, Ambroszkiewicz Jadwiga
Department of Screening Tests and Metabolic Diagnostics, Institute of Mother and Child, Kasprzaka 17a, 01-211 Warsaw, Poland.
Department of Clinical Dietetics, Faculty of Health Sciences, Medical University of Warsaw, E Ciołka Str. 27, 01-445 Warsaw, Poland.
Antioxidants (Basel). 2023 Mar 16;12(3):730. doi: 10.3390/antiox12030730.
Metabolic disorders present in women with polycystic ovary syndrome (PCOS) and the associated risk of obesity may result in increased oxidative stress and reproductive failure. Therefore, we evaluated the concentrations of reduced glutathione (GSH), oxidized glutathione (GSSG), glutathione peroxidase (GPx), and reductase (GR), as well as nuclear factor erythroid 2-related factor 2 (Nrf2) and Kelch-like ECH-associating protein1 (Keap1) in the serum of 56 women with PCOS divided according to the visceral to subcutaneous fat surface ratio (VAT/SAT) and waist-to-hip ratio (WHR) values. Antioxidant parameter levels were measured by competitive inhibition enzyme immunoassay technique. As the VAT/SAT ratio and WHR increased, we observed significantly higher concentrations of GSSG and Keap1 protein and a lower value of the GSSG/GSH ratio (R-index), which is considered an index of cellular redox ( < 0.05). Negative correlations were found between the R-index and body weight, BMI, WHR, subcutaneous and visceral fat surface and the VAT/SAT ratio, and total body fat; positive links were found with fat free mass and total body water. Opposite associations were noted between GSSG level and the aforementioned body composition parameters. Oxidative stress characterized by a depleted reduced-to-oxidized glutathione index is associated with anthropometric and body composition parameters in women with PCOS. In particular, abdominal obesity expressed by the VAT/SAT ratio and/or WHR seems to have a negative impact on glutathione status, which may lead to a disruption of many biological cell processes. The observed negative association of Keap1 with R-index suggests that the elevated oxidative changes dependent on the VAT/SAT ratio may lead to Nrf2 activation to promote antioxidant enzyme expression. Although the GSH/GSSG index as well as the VAT/SAT ratio appear to be good indicators of oxidative status, studies on a larger group of patients should continue to confirm these links among women with PCOS.
多囊卵巢综合征（PCOS）女性存在的代谢紊乱及相关肥胖风险可能会导致氧化应激增加和生殖功能衰竭。因此，我们评估了56名PCOS女性血清中还原型谷胱甘肽（GSH）、氧化型谷胱甘肽（GSSG）、谷胱甘肽过氧化物酶（GPx）和还原酶（GR）的浓度，以及核因子红细胞2相关因子2（Nrf2）和 Kelch样ECH相关蛋白1（Keap1）的浓度，这些女性根据内脏与皮下脂肪表面比率（VAT/SAT）和腰臀比（WHR）值进行分组。通过竞争抑制酶免疫测定技术测量抗氧化参数水平。随着VAT/SAT比率和WHR的增加，我们观察到GSSG和Keap1蛋白的浓度显著升高，而GSSG/GSH比率（R指数）的值较低，该指数被认为是细胞氧化还原的指标（P<0.05）。R指数与体重、BMI、WHR、皮下和内脏脂肪表面、VAT/SAT比率以及全身脂肪之间呈负相关；与去脂体重和全身水分呈正相关。GSSG水平与上述身体成分参数之间存在相反的关联。以还原型与氧化型谷胱甘肽指数降低为特征的氧化应激与PCOS女性的人体测量和身体成分参数相关。特别是，以VAT/SAT比率和/或WHR表示的腹部肥胖似乎对谷胱甘肽状态有负面影响，这可能导致许多生物细胞过程的破坏。观察到的Keap1与R指数的负相关表明，依赖于VAT/SAT比率的氧化变化升高可能导致Nrf2激活，从而促进抗氧化酶的表达。尽管GSH/GSSG指数以及VAT/SAT比率似乎是氧化状态的良好指标，但对更大规模患者群体的研究应继续进行，以证实PCOS女性中这些关联。
