Rinaldi Federica, Del Favero Elena, Moeller Johannes, Hanieh Patrizia Nadia, Passeri Daniele, Rossi Marco, Angeloni Livia, Venditti Iole, Marianecci Carlotta, Carafa Maria, Fratoddi Ilaria
Center for Life Nano Science@Sapienza, Istituto Italiano di Tecnologia (ITT), 00161 Rome, Italy.
Department of Medical Biotechnology and Translational Medicine, University of Milan, 20122 Milano, Italy.
Nanomaterials (Basel). 2019 Aug 17;9(8):1177. doi: 10.3390/nano9081177.
Silver nanoparticles (AgNPs) are widely used as antibacterial agents and anticancer drugs, but often their low stability limits their mass production and broad applications. The use of niosomes as a carrier to protect and envelop AgNPs gives a new perspective to solve these problems. In this study, AgNPs were functionalized with sodium 3-mercapto-1-propanesulfonate (3MPS) to induce hydrophilic behavior, improving loading in Tween 20 and Span 20 niosomes (NioTw20 and NioSp20, respectively). Entrapment efficiency was evaluated by UV analyses and is around 1-4%. Dimensions were investigated by means of dynamic light scattering (DLS) (<2R> = 140 ± 4 nm and <2R> = 251 ± 1 nm respectively for NioTw20 + AgNPs and NioSp20 + AgNPs) and were compared with those by atomic force microscopy (AFM) and small angle X ray scattering (SAXS) analyses. Stability was assessed in water up to 90 days, and both in bovine serum and human serum for up to 8 h. In order to characterize the local structure of niosomes, SAXS measurements have been performed on Tween 20 and Span 20 empty niosomes and loaded with AgNPs. The release profiles of hydrophilic probe calcein and lipophilic probe Nile Red were performed in HEPES buffer and in human serum. All these features contribute to conclude that the two systems, NioTw20 + AgNPs and NioSp20 + AgNPs, are suitable and promising in the field of biological applications.
银纳米颗粒（AgNPs）被广泛用作抗菌剂和抗癌药物，但其稳定性低常常限制了它们的大规模生产和广泛应用。使用囊泡作为载体来保护和包裹AgNPs为解决这些问题提供了新的视角。在本研究中，3-巯基-1-丙烷磺酸钠（3MPS）对AgNPs进行功能化处理以诱导亲水性行为，提高其在吐温20和司盘20囊泡（分别为NioTw20和NioSp20）中的负载量。通过紫外分析评估包封率，其约为1%-4%。通过动态光散射（DLS）研究尺寸（NioTw20 + AgNPs和NioSp20 + AgNPs的<2R>分别为140±4 nm和251±1 nm），并与原子力显微镜（AFM）和小角X射线散射（SAXS）分析的结果进行比较。在水中评估稳定性长达90天，在牛血清和人血清中评估长达8小时。为了表征囊泡的局部结构，对吐温20和司盘20空囊泡以及负载AgNPs的囊泡进行了SAXS测量。在HEPES缓冲液和人血清中进行了亲水性探针钙黄绿素和脂溶性探针尼罗红的释放曲线研究。所有这些特征有助于得出结论，NioTw20 + AgNPs和NioSp20 + AgNPs这两个系统在生物应用领域是合适且有前景的。
