College of Chemistry, Chemical Engineering and Biotechnology, Donghua University, Shanghai 201620, China.
National Engineering Research Center for Dyeing and Finishing of Textiles, Donghua University, Shanghai 201620, China.
Mater Sci Eng C Mater Biol Appl. 2019 Oct;103:109821. doi: 10.1016/j.msec.2019.109821. Epub 2019 May 29.
Violacein, a natural violet biopigment with efficient bioactivities from Gram-negative bacteria, possesses good affinity to silk fiber and complexes with silver. In this paper, a new approach involving the surface modification of silk fabrics with violacein for the in-situ synthesis of silver nanoparticles (SNPs) was developed. Violacein is used to modify silk material. Subsequently, silk containing bio-violacein was in situ assembled by silver ions and formed SNPs. Functional silk composites (FSC) containing bio-violacein and SNPs were obtained with effective synergistic antimicrobial effects. FSC were characterized by FT-IR spectroscopy, UV-visible absorption spectroscopy, and scanning electron microscopy/energy dispersive spectroscopy, and X-ray diffraction. Exhaustion and amount of violacein on silk fabric were 65.82% and 0.16 g/g, respectively. SNPs were small particles with irregular shapes and sizes <60-70 nm. Antimicrobial activities of the FSC were evaluated against S. aureus, E. coli, and C. albicans. The silk fabric with violacein possessed good antimicrobial activity against S. aureus, with a bacterial reduction of 81.25%. FSC with violacein combined with SNPs integration exhibited good synergistic properties as excellent antimicrobial activities against S. aureus, E. coli, and C. albicans, with microbial reductions of 99.98%, 99.90%, and 99.85%, respectively. FSC not only exhibited the enhanced antimicrobial effects but also exhibited a broadened antimicrobial range.
天然紫色生物颜料——来自革兰氏阴性菌的细菌素紫，具有高效的生物活性，对丝纤维具有良好的亲和力，并与银形成复合物。本文开发了一种新的方法，即用细菌素紫对丝织物进行表面改性，以原位合成纳米银颗粒（SNPs）。细菌素紫用于修饰丝材料。随后，通过银离子和形成的 SNPs 原位组装含有生物细菌素紫的丝。含有生物细菌素紫和 SNPs 的功能性丝复合材料（FSC）具有有效的协同抗菌效果。FSC 通过傅里叶变换红外光谱、紫外-可见吸收光谱、扫描电子显微镜/能量色散光谱和 X 射线衍射进行了表征。丝织物上的细菌素紫的耗竭量和含量分别为 65.82%和 0.16 g/g。SNPs 是不规则形状和尺寸 <60-70nm 的小颗粒。评估了 FSC 对金黄色葡萄球菌、大肠杆菌和白色念珠菌的抗菌活性。带有细菌素紫的丝织物对金黄色葡萄球菌具有良好的抗菌活性，细菌减少率为 81.25%。带有细菌素紫的 FSC 与 SNPs 集成的协同作用表现出良好的协同性能，对金黄色葡萄球菌、大肠杆菌和白色念珠菌的抗菌活性分别达到 99.98%、99.90%和 99.85%。FSC 不仅表现出增强的抗菌效果，而且表现出更广泛的抗菌范围。
