MOE Engineering Research Center of Forestry Biomass Materials and Bioenergy, Beijing Forestry University, Beijing, 100083, PR China.
GuangXi Key Laboratory of Chemistry and Engineering of Forest Products, College of Chemistry and Chemical Engineering, Guangxi University for Nationalities, Nanning 530006, PR China.
Carbohydr Polym. 2019 Nov 1;223:115127. doi: 10.1016/j.carbpol.2019.115127. Epub 2019 Jul 27.
Pods from a Gleditsia sinensis Lam. tree were collected and the galactomannan content and other properties were determined at their different developmental stages. In green and immature seed, galactomannan was substituted to a great extent with a mannose to galactose (M/G) ratio of 2.4 from crude polysaccharides. During late galactomannan deposition, it was substituted to a lower extent and this ratio increased rapidly, reaching a M/G ratio of 3.1. Average molecular weight (M) of the extracted polysaccharides first increased, reached the maximum (1.19 × 10) at 17 weeks after flowering (WAF), and then decreased. These changes might result from primary galactomannan biosynthesis and from galactose removal by α-galactosidase in the endosperm. The solubility of crude polysaccharides decreased with increased M/G ratio and maximum solubility was more than 89% that collected at 13 WAF. Rheological properties showed that apparent viscosity was largely influenced by the molecular weight and M/G ratio of galactomannans.
从皂荚树中收集荚果，并在不同发育阶段测定半乳甘露聚糖含量和其他性质。在绿色和未成熟的种子中，甘露聚糖很大程度上被甘露糖取代，粗多糖的甘露糖与半乳糖（M/G）比值为 2.4。在晚期半乳甘露聚糖沉积过程中，取代程度较低，该比值迅速增加，达到 3.1。提取多糖的平均分子量（M）先增加，在开花后 17 周（WAF）达到最大值（1.19×10），然后降低。这些变化可能是由于胚乳中原生半乳甘露聚糖的生物合成和 α-半乳糖苷酶对半乳糖的去除所致。粗多糖的溶解度随 M/G 比值的增加而降低，最大溶解度超过 89%，是在 13WAF 时收集的。流变学特性表明，表观粘度主要受半乳甘露聚糖的分子量和 M/G 比值的影响。
