Department of Analytical Chemistry, Regional Centre of Advanced Technologies and Materials, Faculty of Science, Palacký University in Olomouc, 17. listopadu 12, 77146 Olomouc, Czech Republic.
Sorbonne Université, CNRS, Physico-chimie des Électrolytes et Nanosystèmes Interfaciaux, PHENIX, F-75005 Paris, France.
J Chromatogr A. 2020 Jan 4;1609:460433. doi: 10.1016/j.chroma.2019.460433. Epub 2019 Aug 9.
In this work, interactions of carboxylated core shell magnetic nanoparticles with polymyxin B sulfate were studied by connecting capillary electrophoresis with inductively coupled plasma mass spectrometry. The interaction was probed by affinity mode of capillary electrophoresis with 25 mM phosphate buffer at physiological pH. Fe, Fe, Fe, S, and C isotopes were used to monitor the migration of an electroosmotic flow marker and the interaction of the nanoparticles with polymyxin B. The analysis of interaction data showed two distinct interaction regions, one with low polymyxin B concentration, the second with high polymyxin B concentration. These regions differed in the strength of the interaction, 1.49 × 10 M and 1.60 × 10 M, and in the stoichiometry of 0.7 and 3.5, respectively. These differences can be explained by the decrease of electrostatic repulsion between nanoparticles caused by polymyxin B. This is also in agreement with the nanoparticles peak shapes: sharp for low polymyxin B concentrations and broad for high polymyxin B concentrations.
在这项工作中，通过将毛细管电泳与电感耦合等离子体质谱相结合，研究了羧基核壳磁性纳米粒子与硫酸多粘菌素 B 的相互作用。通过毛细管电泳在生理 pH 值下用 25mM 磷酸盐缓冲液以亲和模式探测相互作用。Fe、Fe、Fe、S 和 C 同位素用于监测电渗流标记物的迁移和纳米粒子与多粘菌素 B 的相互作用。相互作用数据的分析表明存在两个不同的相互作用区域，一个区域的多粘菌素 B 浓度较低，另一个区域的多粘菌素 B 浓度较高。这些区域在相互作用强度、1.49×10^M 和 1.60×10^M 以及化学计量比 0.7 和 3.5 方面存在差异。这种差异可以用多粘菌素 B 引起的纳米粒子之间的静电排斥的减小来解释。这也与纳米粒子的峰形一致：低多粘菌素 B 浓度时峰形尖锐，高多粘菌素 B 浓度时峰形较宽。
