Wu Lan, Deng Tong, Wang Chao-Yang, Ren Xue-Qun, Wang Yun-Yun, Zeng Xian-Tao, Geng Pei-Liang
Center for Evidence-Based and Translational Medicine, Zhongnan Hospital of Wuhan University, Wuhan, China.
Department of Stomatology, Zhongnan Hospital of Wuhan University, Wuhan, China.
Front Genet. 2019 Aug 5;10:706. doi: 10.3389/fgene.2019.00706. eCollection 2019.
The current study is aimed at exploring the relationship between chronic periodontitis and serotonin transporter () gene polymorphisms (rs6354 and rs12449783) in the Chinese Han population. This study included a total of 120 patients with chronic periodontitis and 125 healthy control subjects. The gene (rs6354 and rs12449783) was genotyped using oral mucosal tissue with a polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP). Linkage disequilibrium was examined using Haploview. Genotype and allele frequencies were compared between the cases and controls using a χ test. Genotype distribution of the gene polymorphisms rs6354 and rs12449783 in the control group conformed to Hardy-Weinberg equilibrium. The frequency of the AC genotype, the AC + CC genotype and C allele of the rs6354 polymorphism was higher in cases ( < 0.05) vs. the healthy control. The adjusted odds ratio (OR) was 1.910 (95%CI = 1.049-3.476) for the AC genotype, 2.026 (95%CI = 1.115-3.680) for the AC+CC genotype, and 1.875 for the C allele (95%CI = 1.089-3.228. Such an association was particularly strong in women for the AC genotype (OR = 2.167, 95%CI = 1.034-4.542). The genotype and allele frequencies of rs12449783 did not differ between the cases and controls. Haplotype C-C (rs6354-rs12449783) was also more frequent in the cases (OR = 2.372, 95%CI = 1.154-4.875, P = 0.016). Chronic periodontitis is associated with the gene rs6354 polymorphism, as well as rs6354/rs12449783 interaction.
本研究旨在探讨中国汉族人群中慢性牙周炎与血清素转运体（）基因多态性（rs6354和rs12449783）之间的关系。本研究共纳入120例慢性牙周炎患者和125例健康对照者。采用聚合酶链反应-限制性片段长度多态性（PCR-RFLP）方法对口腔黏膜组织中的基因（rs6354和rs12449783）进行基因分型。使用Haploview软件检测连锁不平衡。采用χ检验比较病例组和对照组的基因型和等位基因频率。对照组中rs6354和rs12449783基因多态性的基因型分布符合Hardy-Weinberg平衡。病例组中rs6354多态性的AC基因型、AC + CC基因型和C等位基因频率高于健康对照组（<0.05）。AC基因型的校正比值比（OR）为1.910（95%CI = 1.049 - 3.476），AC + CC基因型为2.026（95%CI = 1.115 - 3.680），C等位基因为1.875（95%CI = 1.089 - 3.228）。这种关联在女性AC基因型中尤为强烈（OR = 2.167，95%CI = 1.034 - 4.542）。rs12449783的基因型和等位基因频率在病例组和对照组之间没有差异。单倍型C-C（rs6354 - rs12449783）在病例组中也更为常见（OR = 2.372，95%CI = 1.154 - 4.875，P = 0.016）。慢性牙周炎与基因rs6354多态性以及rs6354/rs12449783相互作用有关。
