Nakamura Yoshitaka, Fukushima Michiko, Hoshi Seiko, Chatt Amares, Sakata Takashi
Food Science & Technology Research Laboratories, Meiji Co., Ltd., Hachiouji, Tokyo 192-0919, Japan.
Ishinomaki Senshu University, Ishinomaki, Miyagi 986-8580, Japan.
J Nutr Sci. 2019 Jul 24;8:e24. doi: 10.1017/jns.2019.21. eCollection 2019.
To evaluate the daily Se intake of 3- to 5-year-old Japanese children, we used seventy-two urine samples collected from fifty-three children (twenty-seven male and twenty-six female) from two cities in Miyagi prefecture, Japan. For measuring low Se concentrations with high precision, accuracy and rapidity in the 24-h urine samples, we developed an instrumental neutron activation analysis (INAA) method, that is without any chemical separation, using the short-lived Se ( = 17·4 s) nuclide. The estimated Se intake of the fifty-three children was 51·5 (sd 30·2) µg/d (geometric mean: 42·7 µg/d). Ten subjects (three male and seven female), successfully provided 24-h urine samples over two or three consecutive days; their Se intake was 37·4 (sd 5·9) µg/d. Based on the logarithmically transformed data of these ten subjects, the ratio of intra-/inter-individual variances of usual Se intake was 16·7 (28·0/1·7) and geometric mean was 27·7 µg/d. The 5th to 95th percentile of usual Se intake of these ten subjects was 17·5 to 40·4 µg/d, which ranged between the recommended dietary allowance and tolerable upper intake level of Se by the Dietary Reference Intakes for Japanese (2015).
为评估3至5岁日本儿童的每日硒摄入量，我们使用了从日本宫城县两个城市的53名儿童（27名男性和26名女性）收集的72份尿液样本。为了高精度、准确且快速地测量24小时尿液样本中的低硒浓度，我们开发了一种仪器中子活化分析（INAA）方法，即不进行任何化学分离，使用短寿命硒（半衰期 = 17.4秒）核素。这53名儿童的估计硒摄入量为51.5（标准差30.2）μg/d（几何平均值：42.7 μg/d）。10名受试者（3名男性和7名女性）连续两到三天成功提供了24小时尿液样本；他们的硒摄入量为37.4（标准差5.9）μg/d。基于这10名受试者经对数转换的数据，通常硒摄入量的个体内/个体间方差比为16.7（28.0/1.7），几何平均值为27.7 μg/d。这10名受试者通常硒摄入量的第5至95百分位数为17.5至40.4 μg/d，介于日本膳食参考摄入量（2015年）推荐的膳食摄入量和硒的可耐受最高摄入量之间。
