Meng Xiangyong, Li Tingting, Song Teng, Chen Chang, Venkitasamy Chandrasekar, Pan Zhongli, Zhang Huien
College of Environmental Science and Engineering Anhui Normal University Wuhu China.
Department of Biological and Agricultural Engineering University of California Davis Davis, CA.
Food Sci Nutr. 2019 Jul 15;7(8):2556-2564. doi: 10.1002/fsn3.1105. eCollection 2019 Aug.
This research aims to investigate the solubility, structural properties, and immunomodulatory of rice dreg protein (RDP) modified with sodium alginate. The modification was done by wet heating assisted with microwave treatment. The solubility, emulsifying properties at pH 2-12, amino acid composition, molecular weight distribution, circular dichroism (CD) spectroscopy, and FTIR spectra of modified RDP were analyzed and discussed. Results showed that Maillard reaction could significantly enhance the solubility and emulsifying capacity of RDP. Further, an animal model for cyclophosphamide-induced immunodeficiency was designed to evaluate the immunomodulatory effect of modified RDP. It is therefore suggested that modified RDP could improve the immunomodulatory effect of immunosuppressed mice, and the immunomodulation was concentration dependent, being generally enhanced by increased concentrations. This research revealed that glycosylation modification of RDP through Maillard reaction by wet heating assisted with microwave treatment may be successfully applied to improve the physicochemical properties and bioactive benefits of the final product.
本研究旨在探究经海藻酸钠修饰的米渣蛋白（RDP）的溶解性、结构特性及免疫调节作用。修饰通过微波辅助湿热处理完成。对修饰后的RDP的溶解性、pH值为2 - 12时的乳化特性、氨基酸组成、分子量分布、圆二色（CD）光谱及傅里叶变换红外光谱（FTIR）进行了分析和讨论。结果表明，美拉德反应可显著提高RDP的溶解性和乳化能力。此外，设计了环磷酰胺诱导免疫缺陷的动物模型来评估修饰后RDP的免疫调节作用。因此表明，修饰后的RDP可改善免疫抑制小鼠的免疫调节作用，且免疫调节呈浓度依赖性，一般随浓度增加而增强。本研究表明，通过微波辅助湿热处理的美拉德反应对RDP进行糖基化修饰，可能成功应用于改善最终产品的理化性质和生物活性益处。
