Zhang Hongyin, Shao Shuai, Han Rongxin, Zhang Rongrong, Ma Xintong, Wang Miao, Wan Zhiqiang, Zhao Daqing, Yan Mingming
Jilin Ginseng Academy, Changchun University of Chinese Medicine Changchun 131200 P. R. China
School of Pharmaceutical Sciences, Changchun University of Chinese Medicine Changchun 131200 P. R. China.
RSC Adv. 2020 Aug 11;10(49):29555-29566. doi: 10.1039/d0ra03731a. eCollection 2020 Aug 5.
Semen Ziziphi Spinosae ( Mill. var. (Bunge) Hu ex H. F. Chou) is a functional food and a traditional Chinese medicine (TCM) in China. Herein, Semen Ziziphi Spinosae protein (SZSP) was prepared by an alkaline extraction and acid precipitation method, of which the structural, physicochemical, functional and emulsion properties were investigated. Results showed that SZSP contained an ideal amino acid composition. The structural properties of the proteins were characterized using Fourier transform infrared spectroscopy (FTIR), relative fluorescence and circular dichroism (CD) spectroscopy analysis. The electrophoresis profiles showed that the main molecular weight of the protein components was about 10-40 kDa and contained some glycoproteins. Differential scanning calorimetry analysis indicated that the denaturation temperature of SZSP was 110.5 °C. The functional properties showed that SZSP has good water and oil absorption capacity, high emulsifying ability and foaming stability. The overall results suggest that SZSP is a promising protein source for the functional food industry.
酸枣仁（鼠李科鼠李属植物酸枣的种子）是中国的一种功能性食品和传统中药。在此，采用碱提取酸沉淀法制备了酸枣仁蛋白（SZSP），并对其结构、理化、功能和乳化特性进行了研究。结果表明，SZSP含有理想的氨基酸组成。利用傅里叶变换红外光谱（FTIR）、相对荧光和圆二色（CD）光谱分析对蛋白质的结构特性进行了表征。电泳图谱显示，蛋白质组分的主要分子量约为10-40 kDa，且含有一些糖蛋白。差示扫描量热分析表明，SZSP的变性温度为110.5℃。功能特性表明，SZSP具有良好的吸水吸油能力、高乳化能力和泡沫稳定性。总体结果表明，SZSP是功能性食品工业中一种有前景的蛋白质来源。
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