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影响粪肠球菌中pAMβ1向植物乳杆菌转移频率的因素。

Factors affecting transfer frequency of pAM beta 1 from Streptococcus faecalis to Lactobacillus plantarum.

作者信息

Sasaki Y, Taketomo N, Sasaki T

机构信息

Meiji Institute of Health Science, Odawara, Japan.

出版信息

J Bacteriol. 1988 Dec;170(12):5939-42. doi: 10.1128/jb.170.12.5939-5942.1988.

DOI:10.1128/jb.170.12.5939-5942.1988
PMID:3142863
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC211709/
Abstract

Conjugal transfer of streptococcal plasmid pAM beta 1 to Lactobacillus plantarum by a filter mating method was examined under various conditions. The transfer frequency depended greatly on the type, pore size, and side (front or back) of the membrane filter. The passage of sterilized water through the membrane under reduced pressure after donor and recipient cells were trapped on it increased the transfer frequency about 10-fold.

摘要

采用滤膜交配法，在不同条件下检测了链球菌质粒pAMβ1向植物乳杆菌的接合转移情况。转移频率在很大程度上取决于滤膜的类型、孔径以及滤膜的面（正面或背面）。在供体和受体细胞被捕获在滤膜上之后，通过减压使灭菌水通过滤膜，转移频率提高了约10倍。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e148/211709/5692a97cc4b7/jbacter00190-0548-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e148/211709/5692a97cc4b7/jbacter00190-0548-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e148/211709/5692a97cc4b7/jbacter00190-0548-a.jpg

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