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枯草芽孢杆菌在pAMβ1中产生一个主要的特异性缺失。

Bacillus subtilis generates a major specific deletion in pAM beta 1.

作者信息

van der Lelie D, Venema G

机构信息

Department of Genetics, University of Groningen, Haren, The Netherlands.

出版信息

Appl Environ Microbiol. 1987 Oct;53(10):2458-63. doi: 10.1128/aem.53.10.2458-2463.1987.

DOI:10.1128/aem.53.10.2458-2463.1987
PMID:2827570
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC204129/
Abstract

pAM beta 1, a 26.5-kilobase plasmid originally isolated from Streptococcus faecalis, was conjugally transferred from Streptococcus lactis to Bacillus subtilis. No conjugal transfer of pAM beta 1 from B. subtilis to S. lactis was observed. In addition, pAM beta 1 which had been reintroduced in S. lactis after cycling through B. subtilis had lost its conjugal transferability to Streptococcus cremoris, although under the same conditions noncycled pAM beta 1 was transferred at high efficiency. Restriction and Southern blot analyses showed that pAM beta 1 had suffered one major, specific 10.6-kilobase deletion and several minor but also specific deletions in B. subtilis. Comparing the major deletion derivative, delta pAM beta 1, with B. subtilis strains which have been reported to contain pAM beta 1 showed that these strains also contained delta pAM beta 1. Hybridization experiments showed that the deleted fragment was not transposed to the B. subtilis chromosome. Based on the size of the minor deletion derivatives from pAM beta 1, it is suggested that these use a different origin of replication in B. subtilis.

摘要

pAM beta 1是一种最初从粪链球菌中分离出的26.5千碱基质粒，它通过接合作用从乳酸链球菌转移至枯草芽孢杆菌。未观察到pAM beta 1从枯草芽孢杆菌向乳酸链球菌的接合转移。此外，在通过枯草芽孢杆菌循环后重新导入乳酸链球菌的pAM beta 1，失去了其向乳脂链球菌的接合转移性，尽管在相同条件下未循环的pAM beta 1能高效转移。限制性内切酶分析和Southern印迹分析表明，pAM beta 1在枯草芽孢杆菌中发生了一次主要的、特定的10.6千碱基缺失以及几次较小但同样特定的缺失。将主要缺失衍生物delta pAM beta 1与已报道含有pAM beta 1的枯草芽孢杆菌菌株进行比较，结果表明这些菌株也含有delta pAM beta 1。杂交实验表明，缺失片段未转座至枯草芽孢杆菌染色体。根据pAM beta 1的较小缺失衍生物的大小，推测这些衍生物在枯草芽孢杆菌中使用不同的复制起点。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b62b/204129/f932e870fba4/aem00127-0201-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b62b/204129/4799733eb84d/aem00127-0200-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b62b/204129/ee8695c56029/aem00127-0200-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b62b/204129/0f8cabbd4a8a/aem00127-0201-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b62b/204129/f932e870fba4/aem00127-0201-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b62b/204129/4799733eb84d/aem00127-0200-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b62b/204129/ee8695c56029/aem00127-0200-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b62b/204129/0f8cabbd4a8a/aem00127-0201-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b62b/204129/f932e870fba4/aem00127-0201-b.jpg

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本文引用的文献

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TRANSFORMATION OF BIOCHEMICALLY DEFICIENT STRAINS OF BACILLUS SUBTILIS BY DEOXYRIBONUCLEATE.脱氧核糖核酸对枯草芽孢杆菌生化缺陷菌株的转化
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