Department of Spine Surgery, Jining No.1 People's Hospital , Jining, Shandong Province, P.R. China.
Department of Spine Surgery, Affiliated Jining No.1 People's Hospital of Jining Medical University, Jining Medical University , Jining, Shandong Province, P.R. China.
Connect Tissue Res. 2020 Nov;61(6):594-603. doi: 10.1080/03008207.2019.1651847. Epub 2019 Aug 20.
Intervertebral disc degeneration-related diseases are common health problems in the department of orthopedics. However, there is no effective treatment protecting the intervertebral disc from degeneration. Sesamin, a kind of sesame lignans extracted from sesame seed oil, has been proved to inhibit lipopolysaccharide-induced inflammation and extracellular matrix catabolism in rat intervertebral disc in vitro and ex vivo. The present study was designed to investigate the effects of sesamin on lesion-induced intervertebral disc degeneration in vivo. Degeneration of rat tail disc was induced by puncture lesion, followed by intradiscal injection of sesamin. Magnetic resonance imaging (MRI), quantitative real-time polymerase chain reaction, histological analysis, and biochemical analysis were carried out to analyze degeneration progression 2 weeks after surgery. As shown by results, intradiscal injection of sesamin inhibited the MRI signal decrease of nucleus pulposus (NP) in T2-weighted images. The upregulated mRNA expression of MMP-3 and ADAMTS-5 induced by lesion was significantly suppressed by sesamin injection. Sesamin partly protected mRNA expression of Col2a1 and Acan from downregulation. Intradiscal injection of sesamin effectively maintained the normal morphology of disc and inhibited lesion-induced degeneration-related histological changes. Immunohistochemical assay demonstrated that the upregulation of degradative enzymes protein expression and the downregulation of type II collagen expression in NP were suppressed by sesamin. According to biochemical analysis, sesamin significantly inhibited the lesion-induced decrease of proteoglycan content in NP. The present study proved the protective effects of sesamin on lesion-induced intervertebral disc degeneration at an early stage.
椎间盘退变相关疾病是骨科常见的健康问题。然而，目前尚无有效的治疗方法能够保护椎间盘免受退变的影响。芝麻素是从芝麻油中提取的一种芝麻木脂素，已被证明可抑制脂多糖诱导的大鼠椎间盘体外和离体炎症和细胞外基质分解代谢。本研究旨在探讨芝麻素对体内致病变性椎间盘退变的影响。通过穿刺损伤诱导大鼠尾椎间盘退变，然后向椎间盘内注射芝麻素。在手术后 2 周进行磁共振成像（MRI）、实时定量聚合酶链反应、组织学分析和生化分析，以分析退变进展。结果表明，向椎间盘内注射芝麻素可抑制 T2 加权图像中 NP 的 MRI 信号降低。损伤诱导的 MMP-3 和 ADAMTS-5 mRNA 表达上调被芝麻素注射显著抑制。芝麻素部分保护 Col2a1 和 Acan 的 mRNA 表达免受下调。向椎间盘内注射芝麻素可有效维持椎间盘的正常形态并抑制损伤诱导的退变相关组织学变化。免疫组织化学检测表明，NP 中降解酶蛋白表达的上调和 II 型胶原表达的下调被芝麻素抑制。根据生化分析，芝麻素显著抑制了 NP 中蛋白聚糖含量因损伤而降低。本研究证明了芝麻素对早期致病变性椎间盘退变的保护作用。
