Ferro Pedro, Ortega-Pinazo Jesús, Martínez Beatriz, Jiménez Álvaro, Gómez-Zumaquero Juan M, Hortas Maria L, Díaz Tatiana
Andalusian Public Health System Biobank, Instituto de Investigación Biomédica de Málaga (IBIMA), Malaga, Spain.
Neuroimmunology and Neuroinflammation Group, Instituto de Investigación Biomédica de Málaga (IBIMA), UGC Neurociencias, Hospital Regional Universitario de Málaga, Malaga, Spain.
Biopreserv Biobank. 2019 Dec;17(6):577-582. doi: 10.1089/bio.2019.0024. Epub 2019 Aug 20.
Human biobanks are collections of biological samples and health information that allow the organization of biomedical research for upgrading the knowledge of human disorders from different diseases (cancer, allergies, rare diseases, etc.), and reach real answers for diagnosis and treatment. A wide range of samples can be stored in these biorepositories such as hair, nails, urine, tissue, whole blood, red blood cells, buffy coat, plasma, serum, DNA, and RNA. Among these, buffy coat and whole blood are widely used by researchers because they can obtain DNA and RNA from these matrices. Some preliminary studies have been performed on animals to evaluate the quality and functionality of the nucleic acids obtained from some of these matrices, although more in-depth studies are needed in this area. In this study, blood samples extracted by venipuncture from 30 healthy volunteers were used to obtain DNA from buffy coat and whole blood. The purity and integrity of the nucleic acids obtained were assessed by spectrophotometry, fluorimetry, and agarose electrophoresis, and functionality was assessed by PCR and real-time PCR. Another aspect tested in this study was based on the comparison between short-term and long-term storage at -80°C and fresh samples from both matrices to evaluate the storage conditions at the biobank. Results showed differences in the yield obtained from both matrices as a function of the storage time, although the functionality of all the obtained DNA remained intact.
人类生物样本库是生物样本和健康信息的集合，它有助于组织生物医学研究，以提升对不同疾病（癌症、过敏、罕见病等）引起的人类疾病的认识，并为诊断和治疗找到切实的答案。这些生物样本库可以存储多种样本，如毛发、指甲、尿液、组织、全血、红细胞、血沉棕黄层、血浆、血清、DNA和RNA。其中，血沉棕黄层和全血被研究人员广泛使用，因为他们可以从这些样本中获取DNA和RNA。虽然在这一领域还需要更深入的研究，但已经对动物进行了一些初步研究，以评估从其中一些样本中获得的核酸的质量和功能。在本研究中，从30名健康志愿者静脉穿刺采集的血样被用于从血沉棕黄层和全血中获取DNA。通过分光光度法、荧光法和琼脂糖电泳评估所获得核酸的纯度和完整性，并通过PCR和实时PCR评估其功能。本研究测试的另一个方面是基于在-80°C下短期和长期储存的样本与两种样本的新鲜样本之间的比较，以评估生物样本库的储存条件。结果显示，两种样本的产量随储存时间而有所不同，尽管所有获得的DNA的功能保持完整。
