Barrow E S, Reisner H M, Graham J B
Br J Haematol. 1979 Jul;42(3):455-68. doi: 10.1111/j.1365-2141.1979.tb01154.x.
The three activities associated with factor VIII--coagulant (VIII:C), antigenic (VIIIR:Ag), and platelet agglutinating or Willebrand factor (VIIIR:WF)--have been separated by sequential antibody affinity chromatography, utilizing a rabbit antibody to factor VIII and a spontaneous human antibody to VIII:C. Normal plasma differentially lost its factor VIII-related antigen following passage over the rabbit antibody column. Subsequent passage of the VIIIR:Ag-depleted plasma over the human antibody column resulted in the loss of VIII:C activity, with retention of the Willebrand factor activity, antigen being partially recovered from the heterologous antibody column. These experiments demonstrate that it is possible to separate two of the factor VIII activities, VIIIR:Ag and VIIIR:WF, which are usually regarded as properties of a single molecule.
与凝血因子VIII相关的三种活性——凝血活性（VIII:C）、抗原活性（VIIIR:Ag）以及血小板凝集活性或血管性血友病因子（VIIIR:WF）——已通过连续抗体亲和层析法得以分离，该方法利用了针对凝血因子VIII的兔抗体以及针对VIII:C的一种自发产生的人抗体。正常血浆在通过兔抗体柱后，其与凝血因子VIII相关的抗原出现差异性丢失。随后，将去除了VIIIR:Ag的血浆通过人抗体柱，结果导致VIII:C活性丧失，而血管性血友病因子活性得以保留，抗原可从异源抗体柱中部分回收。这些实验表明，通常被视为单一分子特性的两种凝血因子VIII活性，即VIIIR:Ag和VIIIR:WF，是有可能被分离的。
