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巴拿马中部嗜人按蚊亚科白蛉(双翅目:毛蠓科)的分子验证

Molecular validation of anthropophilic Phlebotominae sandflies (Diptera: Psychodidae) in Central Panama.

作者信息

Dutari Larissa, Loaiza Jose R

机构信息

Instituto de Investigaciones Científicas y Servicios de Alta Tecnología, Ciudad del Saber, República de Panamá.

Acharya Nagarjuna University, Department of Biotechnology, Guntur, India.

出版信息

Mem Inst Oswaldo Cruz. 2019;114:e190034. doi: 10.1590/0074-02760190034. Epub 2019 Aug 15.

DOI:10.1590/0074-02760190034
PMID:31433005
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC6697409/
Abstract

Six Phlebotominae sand fly species are incriminated as biological vectors of human pathogens in Panama, but molecular corroboration is still needed. We aim at confirming the identity of Phlebotominae species documented as anthropophilic in Panama. Adult sandflies were collected from August 2010 to February 2012 in Central Panama using CDC light traps. Species confirmation was accomplished through molecular barcodes and allied sequences from GenBank. A total of 53,366 sand fly specimens representing 18 species were collected. Five species were validated molecularly as single phylogenetic clusters, but Psychodopygus thula depicted two genetically divergent lineages, which may be indicative of cryptic speciation.

摘要

在巴拿马,六种白蛉属沙蝇被认为是人类病原体的生物传播媒介,但仍需分子证据加以证实。我们旨在确认巴拿马记录的嗜人白蛉属物种的身份。2010年8月至2012年2月期间,在巴拿马中部使用疾控中心诱蚊灯诱捕成年沙蝇。通过分子条形码和来自GenBank的相关序列完成物种确认。共收集到代表18个物种的53366只沙蝇标本。五个物种在分子水平上被确认为单一的系统发育簇,但图拉按蚊显示出两个遗传上不同的谱系,这可能表明存在隐存种化现象。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5d99/6697409/bb7f1ec97f9b/1678-8060-mioc-114-e190034-gf.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5d99/6697409/bb7f1ec97f9b/1678-8060-mioc-114-e190034-gf.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5d99/6697409/bb7f1ec97f9b/1678-8060-mioc-114-e190034-gf.jpg

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