Shmeleva E A, Kondrashina N N, Baturina I G
Antibiot Khimioter. 1988 Aug;33(8):601-5.
The total protein fraction was obtained by extraction with nonionic detergent NP-40. The fraction composition and molecular parameters were studied with DDS-Na electrophoresis in polyacryl amide gel. Common properties of the Corynebacterium diphtheriae protein fraction 66 kD and an analogous fraction of the cell walls of staphylococci and the measles virus were shown with immunoblotting. Cell-mediated mechanisms of the immune response to contact with antigens of Corynebacterium diphtheriae cell walls were revealed.
通过用非离子去污剂NP - 40提取获得总蛋白组分。采用聚丙烯酰胺凝胶中的DDS - Na电泳研究该组分的组成和分子参数。通过免疫印迹显示了白喉棒状杆菌66kD蛋白组分以及葡萄球菌细胞壁和麻疹病毒类似组分的共同特性。揭示了针对白喉棒状杆菌细胞壁抗原接触的细胞介导免疫反应机制。
