Azuma I, Kanetsuna F, Taniyama T, Yamamura Y, Hori M
Biken J. 1975 Mar;18(1):1-13.
The adjuvant activity of cell wall skeletons (mycolic acid-arabino-galactan-mucopeptide, CWS) prepared from the cells of mycobacteria, nocardia and corynebacteria was examined in vivo in mice and guinea pigs. The cell wall skeletons of Mycobacterium bovis BCG (BCG-CWS), Nocardia asteroides 131 and Corynebacterium diphtheriae PWC suspended in Freund's incomplete adjuvant (FIA) as water-in-oil emulsions showed potent adjuvant activity on the formation of circulating antibody and cell-mediated immunity to bovine serum albumin (BSA), sheep erythrocytes (SRBC) and sulfanylazo-bovine serum albumin (SA-BSA) in mice and guinea pigs. After acetylation or acid treatment, BCG-CWS retained its adjuvant activity, but the activity of BCG-CWS was destroyed completely by alkaline treatment. The cell wall constituents, arabinose-mycolate and arabino-galactan, prepared from BCG-CWS showed no adjuvant activity. It was also shown that BCG-CWS suspended in phosphate buffered saline or associated with oil droplets augmented cell-mediated cytotoxicity in which thymus-derived lymphocytes (T-cells) are effector cells.
对从分枝杆菌、诺卡氏菌和棒状杆菌细胞制备的细胞壁骨架（霉菌酸-阿拉伯半乳聚糖-粘肽，CWS）的佐剂活性在小鼠和豚鼠体内进行了检测。悬浮于弗氏不完全佐剂（FIA）中形成油包水乳剂的牛型结核分枝杆菌卡介苗（BCG-CWS）、星形诺卡氏菌131和白喉棒状杆菌PWC的细胞壁骨架，对小鼠和豚鼠体内针对牛血清白蛋白（BSA）、绵羊红细胞（SRBC）和硫氢基偶氮牛血清白蛋白（SA-BSA）的循环抗体形成和细胞介导免疫表现出强大的佐剂活性。乙酰化或酸处理后，BCG-CWS保留其佐剂活性，但BCG-CWS的活性经碱处理后完全被破坏。从BCG-CWS制备的细胞壁成分阿拉伯糖霉菌酸和阿拉伯半乳聚糖未表现出佐剂活性。还表明，悬浮于磷酸盐缓冲盐水或与油滴结合的BCG-CWS增强了细胞介导的细胞毒性，其中胸腺来源的淋巴细胞（T细胞）是效应细胞。
