Oral Medicine, Oral and Maxillofacial Pathology, Department of Stomatology II, University of the Basque Country/ EHU, Leioa, 48940 Bizkaia, Spain,
Med Oral Patol Oral Cir Bucal. 2019 Sep 1;24(5):e610-e614. doi: 10.4317/medoral.23020.
To validate oral exfoliative cytology in the analysis of the microRNA expression profile in Oral Lichenoid Disease (OLD).
The expression of 13 microRNAs identified and presented by our group in a previous study was analyzed in 26 cases, 16 diagnosed as OLD and 10 controls with no oral mucosal pathology. Cytological samples from the oral mucosa obtained using an Orcellex toothbrush were analyzed using RT-qPCR and TaqMan microRNA assays.
The aberrant expression was validated for 2 microRNAs (miR-146a-5p and miR-7-1-3p) of those previously recognized in the biopsy study.
This is the first time that oral exfoliative cytology is validated in a study of the alterations of the expression of microRNAs in OLD. The alteration of miR-146a and miR-7 compared to controls was validated. These microRNAs are associated with both inflammatory and carcinogenic phenomena that are involved in the etiopathogenesis of this potentially malignant oral disorder.
验证口腔脱落细胞学在口腔扁平苔藓（OLD）中 microRNA 表达谱分析中的作用。
分析了本研究小组之前的一项研究中确定和提出的 13 种 microRNA 在 26 例病例中的表达情况，其中 16 例被诊断为 OLD，10 例为无口腔黏膜病的对照。使用 Orcellex 牙刷从口腔黏膜获得细胞学样本，使用 RT-qPCR 和 TaqMan microRNA 检测进行分析。
验证了之前在活检研究中发现的 2 种 microRNA（miR-146a-5p 和 miR-7-1-3p）的异常表达。
这是首次在研究 OLD 中 microRNA 表达变化时验证口腔脱落细胞学。与对照组相比，miR-146a 和 miR-7 的变化得到了验证。这些 microRNAs 与炎症和致癌现象有关，这些现象涉及这种潜在恶性口腔疾病的发病机制。
