School of Biological Science and Engineering, Shaanxi University of Technology, Hanzhong, Shaanxi 723001, China.
Genes (Basel). 2019 Aug 20;10(8):628. doi: 10.3390/genes10080628.
The chicken is a common type of poultry that is economically important both for its medicinal and nutritional values. Previous studies have found that free-range chickens have more skeletal muscle mass. The methyltransferase-like 21C gene ( plays an important role in muscle development; however, there have been few reports on the role of in chickens. In this study, we performed a genome-wide identification of chicken genes and analyzed their phylogeny, transcriptional expression profile, and real-time quantitative polymerase chain reaction (qPCR). We identified 10 genes from chickens, 11 from mice, and 32 from humans, and these genes were divided into six groups, which showed a large amount of variation among these three species. A total of 15 motifs were detected in genes, and the intron phase of the gene structure showed that the gene family was conservative in evolution. Further, both the transcript data and qPCR showed that a single gene's ( expression level increased with the muscle development of chickens, indicating that the genes are involved in the development of chicken muscles. Our results provide some reference value for the subsequent study of the function of
鸡是一种常见的家禽，其药用和营养价值都非常重要。先前的研究发现，散养的鸡具有更多的骨骼肌。甲基转移酶样 21C 基因（ ）在肌肉发育中起着重要作用；然而，关于在鸡中的作用的报道很少。在这项研究中，我们进行了鸡 基因的全基因组鉴定，并分析了它们的系统发育、转录表达谱和实时定量聚合酶链反应（qPCR）。我们从鸡中鉴定出 10 个基因，从老鼠中鉴定出 11 个基因，从人类中鉴定出 32 个基因，这些基因分为六个组，在这三个物种中表现出大量的变异。在 基因中总共检测到 15 个基序，基因结构的内含子相位表明该 基因家族在进化中是保守的。此外，转录数据和 qPCR 都表明单个基因的（ 表达水平随着鸡肌肉的发育而增加，表明 基因参与了鸡肌肉的发育。我们的结果为随后研究 基因在鸡肌肉发育中的功能提供了一些参考价值。
