Genome-Wide Identification, Characterization, and Expression Analysis Related to Low-Temperature Stress of the Gene Family in L.

Germin-like protein (GLP) participates in plant growth and development and plays an important role in plant stress. In the present study, 22 belonging to five classes were identified in the melon genome. Each member of the CmGLPs family contains a typical Cupin_1 domain. We conducted a genome-wide analysis of the melon gene family characterization. were randomly distributed in the melon chromosomes, with the largest number on chromosome 8, having eight family members. Gene duplication events drive the evolution and expansion of the melon gene family. Based on the phylogenetic tree analysis of GLP proteins in melon, rice, Arabidopsis, and cucumber, it was found that the gene families of different species have diverged in evolution. Based on qRT-PCR results, all members of the gene family could be expressed in different tissues of melon. Most genes were up-regulated after low-temperature stress. The relative expression of increased by 157.13 times at 48 h after low-temperature treatment. This finding suggests that the might play an important role in low-temperature stress in melon. Furthermore, quantitative dual LUC assays indicated that and can bind the promoter fragment of the . These results were helpful in understanding the functional succession and evolution of the melon gene family and further revealed the response of to low-temperature stress in melon.