Dobrovic A, Trainor K J, Morley A A
Department of Hematology, Flinders University Medical Centre, Bedford Park, South Australia.
Blood. 1988 Dec;72(6):2063-5.
The bcr-abl translocation characteristic of chronic myeloid leukemia (CML) was detected by the polymerase chain reaction (PCR) modified to use mRNA as the starting material. Amplification of a sequence spanning the bcr-abl junction was obtained by using peripheral blood cells from all of 20 patients with classic CML, one patient with acute lymphoblastic leukemia probably secondary to CML, and two cell lines derived from patients with CML. The presence of bcr exon 3 in the mRNA was determined from the size of the amplified sequence; it was present in 14 and absent in seven patients. One leukemic cell per 1,000 nonleukemic cells could be readily detected, thus indicating the great sensitivity of the method. This technique is of routine value in CML both for diagnosis and for following the course of treatment.
通过将聚合酶链反应（PCR）改进为以mRNA作为起始材料，检测出慢性粒细胞白血病（CML）的bcr-abl易位特征。使用20例典型CML患者、1例可能继发于CML的急性淋巴细胞白血病患者的外周血细胞以及2株源自CML患者的细胞系，获得了跨越bcr-abl连接点的序列扩增。根据扩增序列的大小确定mRNA中bcr外显子3的存在情况；14例患者中存在，7例患者中不存在。每1000个非白血病细胞中1个白血病细胞能够轻易被检测到，从而表明该方法具有很高的灵敏度。这项技术在CML的诊断和治疗过程监测中具有常规价值。
