Mohsenzadeh Mehdi, Tabibnejad Nasim, Vatanparast Mahboubeh, Anbari Fatemeh, Ali Khalili Mohammad, Karimi-Zarchi Mojgan
Research and Clinical Center for Infertility, Shahid Sadoughi University of Medical Sciences, Yazd, Iran.
Gerash Al-Zahra Fertility Center, Gerash University of Medical Sciences, Gerash, Iran.
Int J Reprod Biomed. 2019 May 5;17(3):175-84. doi: 10.18502/ijrm.v17i3.4516. eCollection 2019 Mar.
In vitro maturation (IVM) of immature oocytes retrieved from ovarian tissue has been considered as a valuable approach for fertility preservation in cancerous patients.
To evaluate the efficacy of vitrification on oocyte maturation, survival rates, as well as the subcellular oocyte quality post IVM.
The ovarian cortexes from 19 women with cervix and uterine malignancy aged 21-39 yr were collected. Cumulus-oocyte complexes were aspirated from all visible antral follicles. 102 immature oocytes were collected, and 43 oocytes were detected appropriately for IVM (control group). Also, 59 immature oocytes were vitrified, then matured in vitro (IVM) in two groups: with (GDF9) (group 1) and without GDF9 (group 2) supplementation. Rates of oocytes viability, maturation, and survival along with meiotic spindle visualization and zona pellucida birefringence were assessed with Polyscope.
The rate of maturation was significantly higher in controls (55.8%) compared to the other groups. Maturation rate was 23.3% in oocytes cultured in IVM medium enriched with GDF9, and 27.6% in those cultured in IVM medium lacking GDF9 (p = 0.86). Also, the meiotic spindle was present in 74.4% of control oocytes which was significantly higher than the other groups. The proportion of high zona pellucida birefringence was higher in the controls when compared with group 1 (51.2% vs. 23.3%, respectively, p = 0.04).
Vitrification had a detrimental effect on oocyte maturation, viability as well as the subcellular quality of the oocytes after IVM in cancerous women.
从卵巢组织中获取的未成熟卵母细胞进行体外成熟（IVM）被认为是癌症患者生育力保存的一种有价值的方法。
评估玻璃化对卵母细胞成熟、存活率以及体外成熟后卵母细胞亚细胞质量的影响。
收集19名年龄在21 - 39岁患有宫颈和子宫恶性肿瘤的女性的卵巢皮质。从所有可见的窦卵泡中吸出卵丘 - 卵母细胞复合体。共收集到102个未成熟卵母细胞，其中43个卵母细胞被适当检测用于体外成熟（对照组）。另外，59个未成熟卵母细胞被玻璃化，然后分为两组进行体外成熟（IVM）：添加生长分化因子9（GDF9）（第1组）和不添加GDF9（第2组）。使用多聚显微镜评估卵母细胞的活力、成熟率和存活率，以及减数分裂纺锤体可视化和透明带双折射情况。
与其他组相比，对照组的成熟率显著更高（55.8%）。在添加GDF9的IVM培养基中培养的卵母细胞成熟率为23.3%，在不添加GDF9的IVM培养基中培养的卵母细胞成熟率为27.6%（p = 0.86）。此外，74.4%的对照卵母细胞存在减数分裂纺锤体，这显著高于其他组。与第1组相比，对照组中高透明带双折射的比例更高（分别为51.2%和23.3%，p = 0.04）。
玻璃化对癌症女性体外成熟后卵母细胞的成熟、活力以及亚细胞质量有不利影响。
