Safian Fereshteh, Khalili Mohammad Ali, Karimi-Zarchi Mojgan, Mohsenzadeh Mehdi, Ashourzadeh Sareh, Omidi Marjan
Department of Biology and Anatomical Sciences, Shahid Sadoughi University of Medical Sciences, Yazd, Iran.
Department of Biology and Anatomical Sciences, Shahid Sadoughi University of Medical Sciences, Yazd, Iran. ; Research and Clinical Center for Infertility, Shahid Sadoughi University of Medical Sciences, Yazd, Iran.
Iran J Reprod Med. 2015 Aug;13(8):507-12.
In vitro maturation (IVM) of immature oocytes collected from ovary has been proposed for fertility preservation. In addition, quality of oocytes post IVM is one of the factors determining its developmental competence. By using the non-invasive Polscope system, both meiotic spindle (MS) and zona pellucida (ZP) can be assessed in living oocytes.
The aim was to investigate the developmental potential of immature oocytes retrieved from ovarian tissue after IVM, as a method for fertility preservation, in patients with gynecological diseases.
The ovarian cortex from 26 patients with malignant and benign diseases (21-45 years old), were obtained directly from collaborating hospitals, and transported to the IVF center on ice. In total 61 immature oocytes were aspirated, of which 18 (29.5%) were degenerated and discarded. The remaining 43 (70.5%) healthy oocytes were cultured in IVM culture media for 48 hr. The rate of maturity was assessed, and the ZP birefringence and MS were imaged with Polscope technology.
Overall 43 immature oocytes underwent IVM technology, of which 30.2% reached viable metaphase II (MII) oocytes. The ovarian tissues of 9 (34.6%) women were lacking oocytes at any stage. During polarized light microscopy examination, MS could be visualized only in one of the MII oocytes, but high ZP birefringence's were observed in the majority of the oocytes post IVM (61.5%).
Oocytes maturation post IVM from unstimulated ovaries showed a good developmental competence in gynecologic patients. Further studies should be performed to advance the oocyte maturation program, such as co-culture system, for fertility preservation.
从卵巢采集未成熟卵母细胞进行体外成熟（IVM）已被提出用于生育力保存。此外，IVM后卵母细胞的质量是决定其发育能力的因素之一。通过使用非侵入性的偏振光显微镜系统，可以在活卵母细胞中评估减数分裂纺锤体（MS）和透明带（ZP）。
研究从妇科疾病患者卵巢组织中获取的未成熟卵母细胞经IVM后作为生育力保存方法的发育潜力。
从合作医院直接获取26例年龄在21 - 45岁的恶性和良性疾病患者的卵巢皮质，并在冰上运至体外受精中心。共吸出61个未成熟卵母细胞，其中18个（29.5%）退化并丢弃。其余43个（70.5%）健康卵母细胞在IVM培养基中培养48小时。评估成熟率，并用偏振光显微镜技术对ZP双折射和MS进行成像。
总体而言，43个未成熟卵母细胞接受了IVM技术，其中30.2%发育为有活力的中期II（MII）卵母细胞。9名（34.6%）女性的卵巢组织在任何阶段都没有卵母细胞。在偏振光显微镜检查中，仅在一个MII卵母细胞中可观察到MS，但在IVM后的大多数卵母细胞中观察到高ZP双折射（61.5%）。
未刺激卵巢经IVM后的卵母细胞成熟在妇科患者中显示出良好的发育能力。应进一步开展研究以推进卵母细胞成熟方案，如共培养系统，用于生育力保存。
