Jorge Andreia, Pais Alberto, Vitorino Carla
Department of Chemistry, Coimbra Chemistry Centre, University of Coimbra, Coimbra, Portugal.
Faculty of Pharmacy, University of Coimbra, Coimbra, Portugal.
Methods Mol Biol. 2020;2059:259-283. doi: 10.1007/978-1-4939-9798-5_14.
Efficient intracellular delivery of small-interfering ribonucleic acid (siRNA) to the target organ or tissues in the body is assumed as the main hurdle for a widespread use of siRNAs in the clinics. Solid lipid-based nanoparticles (SLNs) and derivatives can potentially fit this purpose by enabling to overcome the extracellular and intracellular physiological barriers affecting the delivery. For that, rational formulations and rational process designs are needed. This chapter addresses a comprehensive description and critical appraisal of the main production methods of this particular type of lipid nanoparticles and the leading strategies to prompt a targeted delivery of siRNA.
小干扰核糖核酸（siRNA）在体内有效递送至靶器官或组织被认为是siRNA在临床上广泛应用的主要障碍。基于固体脂质的纳米颗粒（SLNs）及其衍生物有可能通过克服影响递送的细胞外和细胞内生理屏障来实现这一目的。为此，需要合理的配方和合理的工艺设计。本章全面描述并批判性评估了这类脂质纳米颗粒的主要生产方法以及促进siRNA靶向递送的主要策略。
