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采用乳液法合成用于递送小干扰RNA的PLGA-脂质杂化纳米颗粒用于递送小干扰RNA的PLGA-聚乙二醇-脂质纳米颗粒

Synthesis of PLGA-Lipid Hybrid Nanoparticles for siRNA Delivery Using the Emulsion Method PLGA-PEG-Lipid Nanoparticles for siRNA Delivery.

作者信息

Wang Lei, Griffel Benjamin, Xu Xiaoyang

机构信息

Otto H. York Department of Chemical, Biological and Pharmaceutical Engineering, New Jersey Institute of Technology, University Heights, Newark, NJ, 07102, USA.

College of Chemistry, Chemical Engineering and Materials Science, Soochow University, 199 Ren-Ai Road, Suzhou Industrial Park, Suzhou, Jiangsu, 215123, China.

出版信息

Methods Mol Biol. 2017;1632:231-240. doi: 10.1007/978-1-4939-7138-1_15.

DOI:10.1007/978-1-4939-7138-1_15

PMID:28730443

Abstract

The effective delivery of small interfering RNA (siRNA) to tumor cells remains a challenge for applications in cancer therapy. The development of polymeric nanoparticles with high siRNA loading efficacy has shown great potential for cancer targets. Double emulsion solvent evaporation technique is a useful tool for encapsulation of hydrophilic molecules (e.g., siRNA). Here we describe a versatile platform for siRNA delivery based on PLGA-PEG-cationic lipid nanoparticles by using the double emulsion method. The resulting nanoparticles show high encapsulation efficiency for siRNA (up to 90%) and demonstrate effective downregulation of the target genes in vitro and vivo.

摘要

将小干扰RNA（siRNA）有效递送至肿瘤细胞仍然是癌症治疗应用中的一项挑战。具有高siRNA负载效率的聚合物纳米颗粒的开发在癌症靶向方面显示出巨大潜力。双乳液溶剂蒸发技术是封装亲水性分子（如siRNA）的有用工具。在此，我们描述了一种基于聚乳酸-羟基乙酸共聚物-聚乙二醇-阳离子脂质纳米颗粒的通用siRNA递送平台，采用双乳液法。所得纳米颗粒对siRNA显示出高封装效率（高达90%），并在体外和体内均显示出对靶基因的有效下调作用。